摘要
目的研究五甲基槲皮素(PMQ)对行自体颈静脉移植的心肌重构大鼠模型的治疗作用。方法 30只SD大鼠随机分为模型组、溶剂组和治疗组,建立大鼠颈部自体静脉移植模型后,每晨分别给予生理盐水、溶剂和25 mg/kgPMQ灌胃,均于第15天开始皮下注射血管紧张素Ⅱ[AngⅡ,288μg/(kg·d)],于21 d后取材。测量心脏指数、左心指数,测量心肌羟脯氨酸含量,免疫组化测量胶原Ⅰ、Ⅲ容积分数(CVF)比值CVFⅠ/Ⅲ;测量新生内膜和中膜的厚度比及面积比。结果 PMQ能显著抑制心肌的肥厚及纤维化,并能抑制移植静脉内膜的增生,而溶剂则无此作用。结论 PMQ对心肌重构及移植静脉内膜增生均有改善作用,有潜力成为冠状动脉旁路移植术(CABG)术后的治疗药物。
Objective To investigate the treatment effect of Pentamethylquercetin(PMQ) on Ang Ⅱ induced cardiac remodeling rats with jugular vein graft.Methods 30 SD rats were randomly assigned to 3 groups with 10 rats in each group.Reversed jugular vein was implanted into the carotid artery and Angiotensin Ⅱ [Ang Ⅱ,288 μg/(kg·d)] was injected subcutaneously daily from the 15th day in all rats.Saline,solvent and PMQ(25 mg/kg) were administrated daily via gavage for 21 days in control group,solvent group and PMQ treatment group.The rats were euthanized on the 21st day,the vein grafts and hearts were harvested.The heart weight index and the left ventricular weight index were measured;myocardial hydroxyproline content was measured;CVFⅠ/Ⅲ was calculated;intimal hyperplasia of the vein grafts was assessed.Results Myocardial hypertrophy and fibrosis were decreased by PMQ and intimal hyperplasia was reduced,the effect was not found in solvent.Conclusion PMQ can improve the cardiac remodeling and neointima hyperplasia of vein graft,so PMQ has the potential to therapy patients with coronary heart disease that have accepted coronary artery bypass grafting(CABG).
出处
《中国医药导报》
CAS
2012年第30期13-15,共3页
China Medical Herald
关键词
五甲基槲皮素
心肌重构
内膜增生
Pentamethylquercetin
Myocardial Remodeling
Intimal Hyperplasia