摘要
目的:研究碳包铁纳米笼壳聚糖微球(Fe@CN-CN)在交变磁场中感应产热规律,观察其对大鼠移植性肝癌(HCC)的磁靶向热疗效果。方法:在体外,观察Fe@CN-CN在高频交变磁场中感应产热规律,确定体内实验条件。36只移植性HCC大鼠随机分为3组(n=12),A组生理盐水组,B组微球组,C组微球热疗组。C组治疗中记录肿瘤区及周围器官温度,7d后,测量肿瘤体积、质量,计算瘤质量抑制率,病理切片观察肿瘤病理变化和微球沉积情况,比较各组治疗效果。结果:不同浓度、不同电流强度条件下磁流体均能在20min内达到最高温度,产热效应随着两者升高而增强。肿瘤区组织温度10min内可达到(42.6±0.20)℃,可恒温维持30min,并显著性高于右肝叶和直肠温度(F=10.925,P=0.000)。C组的瘤质量显著小于其他两组(F=98.830,P=0.000)。C组的瘤质量抑制率为48.5%,是B组的1.5倍;C组的肿瘤体积最小,显著小于其他两组(F=89.086,P=0.000),仅是A组的42.5%;病理切片显示C组和B组肿瘤组织中均见Fe@CN-CN沉积,C组肿瘤组织呈重度坏死,B组呈中度坏死,A组呈轻度坏死。结论:Fe@CN-CN具有良好的感应产热性能,能靶向定位于大鼠移植性HCC,在交变磁场作用下能有效升高肿瘤组织温度,从而产生显著的治疗效果。
OBJECTIVE： To study the heat effect of the Fe@ C nanocage-loaded chitosan nanoparticles （Fe @ CN-CN） under an alternating magnetic field and to observe the therapeutic effect of transplanted liver cancer by the mag- netic target hyperthermia. METHODS： To observed Fe@CN-CN in the high-frequency alternating magnetic field induced heat Production in vitro and in vivo 36 liver transplanted rats were randomly divided into 3 groups （n： 12）, A saline group, B microsphere group, C microspheres hyperthermia group. Tumor area and surrounding organs temperature were recorded during treatment in C group. After 7 days tumor size, weight, tumor weight inhibition rate were calculated and pathological changes and microspheres deposition conditions were compared in treatment groups. RESULTS： In different concentrations of magnetic fluid and different current intensity the Fe@CN-CN fluids can achieve maximum temperature within 20 minutes. Its heat effect increased with the rise of the both. The temperature of tumor area within 10 minutes could reach （42.6＋0.20） ~C and constant temperature could maintain for 30 minutes. It was significantly higher than the temperature of the right hepatic lobe and the temperature of rectum （F： 10. 925, P=0. 000）. Tumor weight in C Group was significantly less than that in the other two groups （F：98. 830, P=0. 000）. Tumor weight inhibition of C group was 48.5 ~, that was 1.5 times the B group; The smallest tumor volume in C group was significantly less than that in the other two groups （F： 89. 086, P： 0. 000） and it was only 42.5 % of A group. Pathology showed that Fe@CN-CN depo- sition were seen in tumor tissue of group B and C, C group showed severe necrosis of tumor tissue and B group showed moderate necrosis while A group showed mild necrosis. CONCLUSION： Fe@CN-CN has a good result of heat perform- ance, can target the transplanted liver cancer directly in rats and in the alternating magnetic field can effectively increase the temperature of tumor tiss
出处
《中华肿瘤防治杂志》
CAS
北大核心
2012年第17期1298-1302,共5页
Chinese Journal of Cancer Prevention and Treatment
基金
广东省自然科学基金(04006966)
广东省名医工程研究项目(200431)
深圳市科技计划(2002-K3-121)
关键词
肝肿瘤
实验性
肿瘤移植
磁力学
电磁场
高温
诱发
肿瘤/治疗
liver neoplasms, experimental
neoplasms transplantation
magnetics
electromagnetic fields
hyper-thermia, induced
neoplasms/therapy
