摘要
目的检测和对比血管内皮生长因子(vascular endothelial growth factor,VEGF)、基质金属蛋白酶-9(Matrix metalloproteinase-9,MMP-9)、增殖细胞核抗原(PCNA)在结直肠癌组织、正常组织中的表达水平,研究三者的相互关系以及临床意义。方法收集78例结直肠癌患者的术后结直肠癌组织样本和正常结直肠组织样本,采用免疫组化SP法检测样本中VEGF、MMP-9和PCNA的表达水平,对其结果进行对比分析。结果 VEGF、MMP-9和PCNA在结直肠癌组织的阳性表达率均高于正常结直肠组织(P<0.05),且与分化程度、浸润深度、Dukes分期和淋巴结转移等临床病理特征相关(P<0.05);VEGF与MMP-9、PCNA,MMP-9与PCNA的表达均呈正相关(P<0.05)。结论结直肠癌组织中VEGF、MMP-9的高表达可促进PCNA的增加,三者的高表达提示结直肠癌的恶化程度高以及有癌组织转移的危险。
Objective To investigate the clinical significance and the relationship between the expression of VEGF, MMP-9 and PCNA in colorectal carcinoma and normal tissue. Methods Collected postoperative colorectal cancer tissue samples and normal colorectal tissue samples of 78 cases of colorectal carcinoma. The expressions of VEGF, MMP-9 and PCNA were detected by immunohistochemical method. Results Positive rates of VEGF, MMP-9 and PCNA were higher in colorectal carcinoma than in normal tissue （ P 〈0.05 ）. There is statistical correlation between the expressions of them and tumor differentiation （ P〈 0.05）, infiltration, Dukes stage, metastasis of lymph node （ P d0.05）. It was positively correlated be- tween VEGF and MMP-9, PCNA. Conclusion High expression of VEGF, MMP-9 in colorectal cancer tis- sue could promote the increase of PCNA. It＇s suggest that the higher expression of VEGF, MMP-9 and PCNA, the more serious of deterioration of co[orectal carcinoma and more likely of cancer metastases.
出处
《结直肠肛门外科》
2012年第4期225-227,共3页
Journal of Colorectal & Anal Surgery
