摘要
为研究红车轴草提取物(RCE)对脂多糖(LPS)应激的断奶仔猪生长性能和炎性介质的影响,本试验选用30头健康、平均体重为(6.86±0.14)kg的(21±1)日龄(杜×长×大)断奶仔猪,按照随机区组设计设5个处理,每个处理6个重复,每个重复1头仔猪.5个处理组仔猪分别饲喂基础日粮(非应激空白对照组)、基础日粮+LPS(LPS组)、基础日粮+LPS+0.05%RCE(0.05%RCE组)、基础日粮+LPS+0.10%RCE(0.10%RCE组)、基础日粮+LPS+0.20%RCE(0.20%RCE组).试验期21 d.在试验第7、14天,LPS组和0.05%、0.10%、0.20%RCE组仔猪每千克体重注射80μg LPS,空白对照组注射等量的生理盐水.结果表明:(1)试验第1-7天(即LPS应激前),与空白对照组、LPS组相比,0.10%RCE组仔猪的日增重显著升高(P<0.05),料重比显著降低(P<0.05),而0.05%和0.20%RCE组的日增重和料重比与空白对照组、LPS组相比无显著差异(P>0.05);试验第7-21天(即注射LPS两次之后),与空白对照组相比,LPS组的日增重和日采食量显著降低(P<0.05),料重比显著提高(P<0.05),而0.05%、0.10%和0.20%RCE组的日增重和日采食量有所下降,但差异不显著(P>0.05).(2)在第7、14天注射LPS后,与空白对照组相比,LPS组仔猪血清中的IgG含量显著降低(P<0.05),血清中白介素-6(IL-6)和肿瘤坏死因子TNF-α的含量均显著提高(P<0.05).与LPS组相比,0.05%RCE组的IgG、IL-6和TNF-α含量差异不显著(P>0.05);0.10%和0.20%RCE组的IgG含量均显著提高P<0.05),IL-6和TNF-α含量均显著降低(P<0.05);以添加0.10%RCE缓解仔猪应激效果最为明显,与LPS组相比,IgG含量提高38.65%(P<0.05),IL-6含量降低32.81%(P<0.05),TNF-α含量降低24.68%(P<0.05).可见,日粮中添加RCE可在一定程度上缓解LPS刺激导致的断奶仔猪生长性能和炎性细胞因子分泌的改变,以添加0.10%RCE效果最好.
To study the effects of red clover extract（REC） on growth performance and inflammatory parameters of weaned pigs with lipopolysaccharide（LPS） challenging.Thirty healthy Duroc×Landrace×Yorkshire crossbred weaned piglets with body weight of（6.86±0.14） kg were randomly allocated to 5 groups.Every group included 6 replicates and 1 piglet in per replicate.The five treatments groups include： basic ration groups（no LPS control groups）,basic ration groups＋LPS control groups（LPS control groups）,basic ration groups＋LPS control groups＋0.05% RCE groups（0.05% RCE groups）,basic ration groups＋LPS control groups＋0.10% RCE groups（0.10% RCE groups）,basic ration groups＋LPS control groups＋0.20% RCE groups（0.20% RCE groups）.On the 21st day after weanling,experiment piglets in groups LPS,0.05% RCE,0.10% RCE and 0.20% RCE were injected intraperitoneally with 80 μg·kg-1 LPS every other day（on day 7,7 and 14 of experiment）,whereas pigs in groups 1 were injected with an equivalent amount of sterile saline.The results showed：（1） Compared with the blank control groups and LPS groups,from day 1 to 7（before LPS challenge）,the average daily gain of piglets in the 0.10% RCE groups increased significantly（P0.05） and average feed-gain ratio decreased significantly（P0.05）.While there was no significant difference among 0.05% RCE groups,0.20% RCE groups and control groups on average daily gain of piglets and feed/gain ratio（P0.05）.From day 7 to 21（with LPS challenge twice）,the average daily gain of piglets and average daily feed intake in LPS groups decreased significantly（P0.05） and average feed-gain ratio increased significantly（P0.05） compared with the blank control groups.While the average daily gain of piglets and average daily feed intake in 0.05% REC groups,0.1% REC groups,0.20% RCE groups decreased,but not significantly（P0.05）.（2） After injected intraperitoneally with LPS every other day（on day 7 and 14 of experiment）,the serumal IgG level
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2012年第5期529-533,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省科技计划重点项目(2010N0023)
龙岩市科技计划项目(2012LY44
2011LY18)
关键词
红车轴草提取物
脂多糖
断奶仔猪
生长性能
炎性介质
red clover extract
lipopolysaccharide
weaned pigs
growth performance
inflammatory parameters
