摘要
试验用大肠杆菌脂多糖(LPS)多次刺激断奶仔猪建立应激模型,研究α-酮戊二酸(AKG)对断奶仔猪的生长性能和血浆激素水平的影响,探讨AKG能否缓解仔猪应激反应。选用24头健康断奶仔猪(杜洛克×长白×大白,体重(7.25±0.23)kg),随机分成3个组(空白对照组、应激对照组和AKG组),每组8个重复,每个重复1头猪。2个对照组饲喂基础日粮+1%淀粉,AKG组饲喂基础日粮+1%AKG,在试验第10天、第12天、第14天和第16天分别在应激对照组和AKG组仔猪的腹膜注射80 μg/kg LPS。试验结果显示:(1)试验第1～9天,未注射LPS时,AKG组平均日增重分别比空白对照组和应激对照组提高30.0%(P<0.05)和31.1%(P<0.05),料重比降低12.4%(P<0.05)和12.8%(P<0.05);试验第10～16天(注射LPS),应激对照组与空白对照组相比平均日增重降低28.8%(P<0.05),料重比升高16.8%(P<0.05),但AKG组的平均日增重和料重比与空白对照组相比无显著性差异(P>0.05);(2)与空白对照组相比,试验第10天,首次LPS刺激后,应激对照组的血浆胰岛素样生长因子(IGF-Ⅰ)水平降低22.7%(P<0.05),而AKG组无显著变化(P>0.05);试验第16天,多次LPS刺激后,与空白对照组比较,应激对照组的IGF-Ⅰ和胰岛素(INS)水平分别降低21.2%(P<0.05)和25.7%(P<0.05),而AKG组无显著变化(P>0.05)。结果表明,日粮中添加1%AKG能显著缓解LPS应激对杜×长×大断奶仔猪生长的负影响。
Constituting a stress model by repeatedly challenged with lipopolysaccharide （LPS） in weaned pigs, the experiment that was researched on effects of α-ketoglutaric acid （AKG） on growth performance and plasma hormones level was aimed at whether AKG could alleviate the reaction to stress in weaned pigs. Twenty-four healthy crossbred Duroc × Landrace × Largewhite weaned piglets with body weight of （7.25 ± 0.23） kg were randomly allocated to 3 groups （control group, LPS control group and AKG group）. Every group included 8 replicates and I piglet in per replicate. The control group and LPS control group were fed the basal ration adding with 1% amylum, while AKG group was fed the basal ration adding with 1% AKG. Piglets in LPS control and AKG group were injected intraperitoneally with 80 μg/kg LPS every other day （on day 10, 12, 14 and 16 of experiment）. The results showed that： 1） from day 1 to day 9 （without LPS challenge）, ADG in AKG group increased by 30.0% （P〈0.05） and 31.1% （P〈0.05） compared with control group and LPS control group, respectively; meanwhile, F/G decreased by 12.4% （P〈0.05） and 12.8% （P〈0.05） ; from day 10 to day 16 （with LPS challenge）, ADG and F/G in LPS control group decreased by 28.8% （P〈0.05） and increased by 16.8% （P〈0.05） compared with control group, respectively; while, there was no significant difference between AKG group and control group on ADG and F/G （P〈0.05） ; 2） in comparison with control group, insulin-like growth factor-Ⅰ （IGF- Ⅰ） of plasma in LPS control group decreased by 22.7% （P〈0.05） after LPS challenged firstly （on day 10 of experiment）, but no significant difference was found in AKG group and control group; after repeated challenge with LPS （on day 16 of experiment）, IGF- I and insulin of plasma in LPS group decreased by 21.2% （P〈0.05）and 25.7% （P〈0.05） ; meanwhile, these indexes had no significant difference between AKG group and control group （PM0.05）. The
出处
《动物营养学报》
CAS
CSCD
北大核心
2009年第4期519-524,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(30871801)
湖北省自然科学基金计划创新群体项目(2007ABC009)
关键词
Α-酮戊二酸
脂多糖
断奶仔猪
生长性能
血浆激素水平
α-ketoglutarate acid
Lipopolysaccharide
Weaned pigs
Growth performance
Plasma hormones level
