摘要
以花生成熟胚培养成的无菌苗叶节为外植体 ,在高浓度 6-BA ( 3 0 mg/ L) MS培养基中 ,光强 2 0 0 0Lx,( 2 5± 3 )℃和 1 4 / 1 0光暗比条件下培养 ,50天后全部诱导分化出整齐一致的丛生芽 ,每个外植体平均产生 8.2 5个丛生芽 ;切下带少许丛生芽的组织块转入含 6-BA ( 3 0 mg/ L) ,LH ( 40 0 mg/ L)和 YE ( 0 .0 1 % )的 MS培养基中 ,可诱导出新的丛生芽 ,诱导率达 98.70 % ,平均出芽 1 3 .86个 .将花生成熟胚轴转接于含NAA ( 1 mg/ L)和 6-BA ( 3 mg/ L) MS培养基中 ,2 0～ 3 0天后 ,诱导出大量正常的体细胞胚 ,诱导率达 95.7% ,将胚切成小块转入含 2 ,4 -D ( 2 0 mg/ L) ,6-BA ( 3 mg/ L )和 LH ( 2 0 0 m g/ L) MS培养基中 ,经 1 5天培养 ,诱导出白色致密的胚性愈伤组织 .
WT5BZ]The single node with leaf explants from shoots cultivated by mature embryos in peanut was plated on MS medium with high concentration of 6 BA(30 mg/L) under 2000 Lx, at (25±3)℃ and 14/10 proportion of light and dark. After 50 days, all explants differentiated normal regular adventitious shoots. There was 8 25 shoots per explant in average. Culturing tissues with some shoots on MS medium with 6 BA(30 mg/L),LH(400 mg/L) and YE(0 01%). New adventitious shoots could be induced with the rate of 98 70% .Per tissue has 13 86 shoots Planting mature embryoid axis in peanut on MS medium containing NAA(1 mg/L) and 6 BA(3 mg/L) for 25～30 days, alot of normal somatic embryos could be induced Somatic embryoid inducing rate was 95 7% Then somatic embryos were separated into some small tissues Transferring them to MS medium containing 2,4 D(20 mg/L), 6 BA(1 mg/L) and LH(200 mg/L), and cultured for 15 days, tight white embryonal calli were well induced.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
2000年第2期75-79,共5页
Journal of Fujian Normal University：Natural Science Edition
基金
福建省教委科研基金资助项目!( K C10 12 )
关键词
花生
体细胞胚
丛生芽
诱导
扩繁培养
Arachis hypogaea
somatic embryos
adventitious shoots
induction
