摘要
目的:建立印度獐牙菜中龙胆苦苷、獐牙菜苷、芒果苷等10种成分的含量测定方法,确定其含量测定指标成分及其限量标准。方法:用Welch materials AQ-C18色谱柱;以甲醇和0.4%甲酸溶液为流动相进行梯度洗脱;柱温30℃,流速1.0mL.min-1,检测波长254 nm。结果:线性关系良好,精密度高,重复性好,回收率均在97%～103%;市场商品中各成分含量变异较大。结论:该方法简便、灵敏、重复性好,适用于印度獐牙菜中各主要成分的含量测定;建议以獐牙菜苷和芒果苷作为"甲蒂(印度獐牙菜)"的含量测定指标成分,其含量限量分别为不得低于0.1%,0.3%。
Objective： To establish a method for determination of l0 ingredients such as gentiopicroside, sweroside, and mangiferin in India swertia, and settle the index components and their limits. Method： By Welch materials AQ-C18 column, determination was conducted by the gradient elution with methanol and 0.4% formic acid as mobile phase, with column temperature 30 ℃, flow rate at 1.0 mL · min-1, and 254 nm as the detection wavelength. Result： The linear relatives of 10 ingredients were good. The method showed the high precision and good reproducibility, and recovery rates were between 97% and 103%. The ingredients of market com- modities varied greatly. Conclusion ： This method is simple, sensitive, reproducible, and applicable to the determination of the main ingredients in India Swertia. Sweroside and mango glycosides were suggested as the index components for determination in Jia Di （ Swertia chirayita） , and their content limits are not less than 0.1% ,0.3 % , respectively.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第20期3141-3146,共6页
China Journal of Chinese Materia Medica
基金
国家科技支撑计划项目子课题(2006DA106A11-12)
创新药物孵化基地项目子课题(2010ZX09401-306-3-9)
关键词
藏药
甲蒂
印度獐牙菜
HPLC
含量测定
含量限量
Tibetan medicine
Jia Di
Swertia chirayita
HPLC, determination
limited content
