摘要
目的:建立藏药"蒂达"不同药材品种的薄层色谱鉴别方法。方法:采用硅胶G板、三氯甲烷-甲醇(40∶1)展开,分离鉴别"蒂达"不同药材品种中的齐墩果酸成分。结果:对70个来源于龙胆科獐牙菜属、花锚属、扁蕾属、肋柱花属及虎耳草科虎耳草属的"蒂达"各品种药材中齐墩果酸的薄层色谱鉴别表明,除虎耳草外,其他来源品种中均检出有齐墩果酸,同种的各样品间薄层图谱特征一致,但同属不同种间及同科不同属间薄层图谱特征存在一定差异。结论:齐墩果酸的薄层鉴别方法可用于"藏茵陈"药材部分种的鉴别,可以有效鉴别来源于虎耳草科与其他种的"蒂达"样品。
Objective： To establish a method of TLC identification for Dida commonly used in Tibetan medicine from different species. Method ： With silica gel G as the stationary phase, and chloroform-methanol （40：1 ） as mobile phase, oleanolic acid from different species of Dida was separated and identified. Result： Oleanolic acid was detected in 70 kinds of Dida derived from the Genti- anaceae Swertia, Halenia, Gentianopsis, Lomatogonium, and Saxifragaceae saxifrage, except for the saxifrage, there are some differ- ences among different genera or subjection. Conclusion： This TLC method can be used for identification of oleanolic acid in Dida from different species except saxifrage.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第5期757-761,共5页
China Journal of Chinese Materia Medica
基金
国家科技支撑计划项目子课题(2006DA106A11-12)
创新药物孵化基地项目子课题(2010ZX09401-306-3-9)
国家科技重大专项子课题(2012ZX09103201-050)
