摘要
目的建立川楝子乙醇提取部位的HPLC指纹图谱。方法色谱柱Agilent zorbax SB-C18柱(4.6 mm×150 mm,5μm);流动相甲醇/乙腈(1∶10)(A)-0.4%磷酸溶液(B),梯度洗脱;检测波长360 nm;柱温25℃;流速1.0 mL.min 1。结果建立了川楝子药材乙醇提取部位的HPLC色谱指纹图谱。在指纹图谱中共有峰20个,并指认了3个色谱峰。结论该方法准确可靠,重复性好,可为川楝子药材及其饮片的质量控制提供参考依据。
OBJECTIVE To establish HPLC fingerprints of ethanolic extraction part of Toosendan Fructus.METHODS Agilent zorbax SB-C18 column（4.6 mm×150 mm,5 μm） was adopted.A gradient elution was applied with mobile phase A（methanol and acetonitrile 1∶10） and mobile phase B（0.4% phosphoric acid solution）.The flow rate was 1.0 mL.min 1,the detective wavelength was 360 nm,and the column temperature was 25 ℃.RESULTS HPLC fingerprints of ethanolic extraction part of Toosendan Fructus was established.Twenty peaks were observed on the HPLC fingerprints of Toosendan Fructus and 3 of them were identified.CONCLUSION The method developed in this study is accurate with high repeatability,and available for the quality control of Toosendan Fructus and its decoction pieces.
出处
《中国现代应用药学》
CAS
CSCD
2012年第9期814-818,共5页
Chinese Journal of Modern Applied Pharmacy
