摘要
目的:建立不同产地川楝子药材的色谱指纹谱,为其质量综合评价提供依据。方法:采用RP-HPLC梯度洗脱技术进行分离分析,色谱柱:Inertsil ODS-3 C18柱;流动相:乙腈-水梯度洗脱;检测波长:270nm;柱温:25℃;流速:1.0mL/min;进样量:20μL。结果:建立了川楝子的色谱指纹谱,制定了控制川楝子质量的技术参数,其中标示39个峰为其特征指纹。结论:不同产地川楝子药材在组分的分布上差异不大,但在成分的比例和量上差异较大。同一产地川楝子在组分的分布和含量上差异较小。该法重现性好,简单,易行,可为川楝子的质量控制提供依据。
AIM： To establish the chromatography fingerprint of the constituents of Melia toosendan Sieb from different areas in order to provide a base for the identification of its quality. METHODS： A gradient separated method was applied. Column ： Inertsil ODS-3 Cls, flow rate ： 1.0 mL/min, column temperature ： 25 ℃. mobile phase ： acetonitrile-water, detection wavelength ： 270 nm, RESULTS ： To establish the chromatography fingerprint of the constituents of Melia toosendan Sieb. , make the technical parameters for its quality controlling, and mark 39 main peaks as its characteristic fingerprint. CONCLUSION： The distribution of constituents of Melia toosendan Sieb differ a little from different areas, but the propotion of the constituents differ greatly, with the Melia toosendan Sieb. from the same area , the distribution and propotion differ a little. This method is reproducible, simple and easy, and can be use to provide a base for the quality control of Melia toosendan Sieb.
出处
《中成药》
CAS
CSCD
北大核心
2009年第2期168-171,共4页
Chinese Traditional Patent Medicine
基金
上海市科学技术委员会科研计划项目(05DZ19736,06DZ22910)
关键词
川楝子
HPLC
色谱指纹谱
Melia toosendan Sieb
HPLC
chromatography fingerprint
