摘要
[目的]采用乙醇提取法,优选香樟根总黄酮的提取工艺。[方法]选择乙醇浓度、料液比、提取温度、提取时间为考察因素,采用正交设计L9(34)进行提取工艺优选试验。[结果]回归方程为:Y=13.68X-0.0027,r2=0.998,芦丁浓度在0.0—0.04516mg/mL范围内有良好线性关系,最佳提取工艺为:乙醇的体积分数为50%,料液比为1:20,温度为70℃,提取时间为2h。[结论]优选的提取工艺简单、易行,可作为开发香樟根制剂的依据。
Objective： Using of ethanol extraction to Optimize extraction technology of total Flavonoids from Cinnamomum camphora roots. Method：Through ethanol concentration , the solid liquid ratio, extraction temperature and extraction time as the study factors, the optimum process for extracting the flavonoids by Orthogonal design L9 （3^4） . Resuhs：The regression equation was Y = 13. 68X -0. 0027 ,r2 =0.99 8 ,confirming a good linear relationship between the concentration and absorbance of rutin in the range of 0. 0 - 0. 04516mg · mL-1 , the optimum conditions of extracting were 50% ethanol, solid liquid ratio is 1：20, extraction temperature is 70℃： , extraction time is 2 h. Conclusion：Optimization of the extraction process is simple and easy , could be used the basis for the development of camphor root preparations.
出处
《贵阳学院学报（自然科学版）》
2012年第3期44-46,共3页
Journal of Guiyang University：Natural Sciences
基金
贵阳学院科研项目(项目编号:2009036)
贵阳市科技局大学生科技创业计划项目(项目合同编号:2011201)
关键词
香樟根
总黄酮
提取
正交试验
Cinnamomum camphora rootes
Total flavonoids
Extraction
Orthogonal test
