摘要
目的:建立常用中药瓜蒌中5-羟甲基糠醛(5-HMF)的高效液相色谱(HPLC)测定方法。方法:采用HPLC法,ODS色谱柱(4.6 mm×250 mm,5μm),甲醇-水(15∶85)为流动相,流速0.8 mL.min-1,检测波长284 nm,柱温30℃。结果:建立了瓜蒌饮片中5-HMF的HPLC测定方法,18批市售瓜蒌样品中5-HMF的含量范围为11.10～193.6μg.g-1。结论:各地的商品瓜蒌中5-HMF的含量差异较大,该方法可作为评价瓜蒌饮片质量的指标之一。
Objective： To establish an HPLC method for content determination of 5-hydroxymethylfurfural （5-HMF） in commercial Trichosanthis Fructus, a commonly used Chinese material medica. Method： The HPLC analysis was performed on an ODS column （4. 6 mm ×250 mm, 5 μm） and the mobile phase consisted of methanol- water （15：85） at a flow rate of 0. 8 mL·min^（-1） The detective wavelength was set at 284 nm and the column temperature was maiutain at 30 ℃. Result： The HPLC method for content determination of 5-HMF in Trichosanthis Fructus was established. 5-HMF was detected in 18 batches of commercial Trichosanthis Fructus, the content of which were in the range of 11.10-193.6 μg ·g^（-1） Conclusion： The content of 5-HMF in commercial Trichosanthis Fructus is quite different. This method is suitable for the quality control of Trichosanthis Fructus.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第18期73-76,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
2011年中医药行业科研专项(201107009)
关键词
瓜蒌
5-羟甲基糠醛
高效液相色谱
含量测定
麦拉德反应
Trichosanthis Fructus
5-hydroxymethylfurfural （5-HMF）
HPLC
content determination
Maillard reaction
