摘要
将黑米糠酶解液用活性炭脱色,研究活性炭添加量、pH、温度和脱色时间对脱色率和多肽回收率的影响,并采用正交试验优化脱色工艺。试验结果表明,黑米糠酶解液的最佳脱色工艺为活性炭添加量0.2%,pH3.0,温度70℃,脱色时间20min。在此条件下,脱色率达96.85%,多肽回收率达81.27%。将脱色酶解液超滤后进行冷冻干燥得酶解物,用DEAE-Sephadex A50离子交换层析初步分离,得到组分AE-F_1和AE-F_2,研究各分离组分和酶解物对DPPH.自由基的清除作用。结果组分AE-F_1的活性最高,清除DPPH.自由基的IC_50为0.93mg/mL。
Black rice bran enzymatic hydrolysate was decolored with activated carbon.The influence of activated carbon addition quantity,pH,temperature and time on the decoloration rate and peptide recovery were studied in the paper by orthogonal methodology.The results showed that the optimal decoloration conditions were as follows：quantity of activated carbon 0.2%,pH 3.0,and temperature 70 ℃ for 20 min.Under these conditions,the decoloration rate and peptide recovery of the hydrolysate reached 96.85% and 81.27% respectively.The decolored hydrolysate was purified using membrane ultrafiltration,then lyophilized and fractionated by anionic exchange chromatography DEAE-Sephadex A50.Then fractions AE-F1 and AE-F2 were obtained.Fractions and lyophilized hydrolysate were assessed using methods of DPPH· radical scavenging ability.It was found that the fraction AE-F1 exhibited the strongest antioxidant activity,with the IC50 value 0.93 mg/mL.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2012年第8期41-46,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
山东省高等学校科技计划项目(J10LC05)
关键词
黑米糠
脱色
抗氧化肽
DPPH·
black rice bran
decolorization
antioxidant peptide
DPPH·
