摘要
以防风为材料,研究不同外植体、激素组合、培养基对愈伤组织诱导及植株再生的影响。结果表明,茎段是防风组织培养较为理想的外植体材料。诱导愈伤组织的最佳培养基为MS+1.5 mg/L 2,4-D+0.5 mg/L KT+1.0 mg/L 6-BA,最高出愈率为96.7%;诱导不定芽的最佳培养基为MS+1.0 mg/L 6-BA+0.5 mg/L NAA,最高诱导率为75%;诱导根的最佳培养基为1/2MS+0.5 mg/L NAA+0.1 mg/L 6-BA,生根率达65%;组培苗的移栽成活率达80%。
Different explants of Saposhnikovia divaricata were used to study the influence of different hormone combinations and media on callus induction and plant regeneration. The results showed that stem seg- ments were the optimal explants; the optimal medium for callus induction was MS ＋ 1.5 mg/L 2,4 -D ＋0.5 mg/L KT ＋ 1.0 mg/L 6 - BA, and on it, the callus forming rate could reach to 96.7% ; the optimal medium for induction of adventitious buds was MS ＋ 1.0 mg/L 6 - BA ＋ 0.5 mg/L NAA, and the highest induction rate was 72%. 1/2MS ＋ 0.5 mg/L NAA ＋ 0.1 mg/L 6 - BA was the best for rooting, and on which, the rooting rate could reach to 65%. The transplanting survival rate of culture seedlings could reach to 80%.
出处
《山东农业科学》
2012年第8期13-16,共4页
Shandong Agricultural Sciences
关键词
防风
组织培养
愈伤组织
Saposhnikovia divaricata
Tissue Culture
Callus
