摘要
目的研究正弦交变电磁场(SEMFs)对体外培养大鼠成骨细胞增殖与分化的影响。方法体外分离培养大鼠颅骨成骨细胞,传代后随机分为6组,分别用频率50 Hz、强度为3.6 mT SEMFs处理,处理时间分别为每天0.5、1.0、1.5、2.0、2.5 h。结果 SEMFs处理后3～5 d,细胞呈现特征样分布。SEMFs处理后9 d,0.5 h组碱性磷酸酶(ALP)活性显著增加,ALP组织化学染色和钙化结节面积结果与ALP活性一致。在SEMFs处理后48 h和96 h,骨保护素和胶原ⅠmRNA表达水平明显高于对照组,尤以0.5 h组最明显。结论 50 Hz、3.6 mT SEMFs可抑制成骨细胞增殖,但能促进体外培养成骨细胞分化成熟。
Objective To investigated the effect of 50-Hz 3.6-mT sinusoidal electromagnetic fields （SEMFs） on the proliferation and differentiation of osteoblasts in vitro. Methods The newborn rat calvarial osteoblasts were isolated by enzyme digestion and randomly divided into 6 groups after one passage. The treat- ment groups under 50-Hz 3.6-roT SEMFs and controls without SEMFs treatment. The cells were exposed in the SEMFs for 0.5 h, 1.0 h, 1.5 h, 2.0 h, and 2.5 h. They were observed under the contrast phase microscope each day. The calcified nodules were stained by alizarin red. The SEMFs were arranged in spiral appearance af- ter 3 to 5 days. Results The SEMFs showed characteristic distribution 3 to 5 days after SEMFs treatment. On the 9th day after treatment, the activity of alkaline phosphatase （ALP） significantly increased in the 0.5-h group, whereas the ALP histochemical straining results and the area of calcified nodules were consistent with ALP activity. In the 48-h and 96-h groups, the genetic expression levels of osteoprotegerin and collagen- I were significantly higher than that in the control group; particularly, the mRNA expression increased in the 0.5-h group. Conclusion The SEMFs at 50-Hz 3.6-mT could suppress the proliferation of osteoblastsmaturation but slimulate the differentiation and maturation of osteoblasts in vitro.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2012年第4期353-358,共6页
Acta Academiae Medicinae Sinicae
基金
甘肃省科技重大专项资助项目(09ZNKDA025)~~
关键词
成骨细胞
正弦交变电磁场
胶原Ⅰ
骨保护素
osteoblasts
sinusoidal electromagnetic fields
collagen-1
osteoprotegerin
