摘要
目的观察BEMF对OVX-OP大鼠骨组织TGF-β1表达的影响,探讨BEMF治疗骨质疏松的机制。方法6月龄雌性未孕Wistar大鼠40只,按体质量随机分为模型组(OVX)、假手术组(Sham)、BEMF治疗组(BEMF+OVX EM)、雌激素治疗组(Estrogen+OVX,E)。OVX、EM、E组行双侧卵巢切除术,Sham组行假手术。术后第9周开始治疗:E组行苯甲酸雌二醇肌肉注射,0.5mg/kg,1次/2周。EM组大鼠暴露于仿生脉冲电磁场治疗,1h.次-1.d-1。OVX、Sham组不予以任何处理,作为对照组。治疗10周后处死各组实验动物,测量腰椎骨密度、椎体的最大载荷、组织学观察椎体骨结构的变化。采用免疫组化检测椎体骨组织中TGF-β1表达情况并以图像分析进行组间比较。结果TGF-β1在骨组织中主要表达于成骨细胞、骨细胞。EM组骨组织TGF-β1的表达较OVX组明显增强,差异有统计学意义(P<0.05);骨结构较对OVX组明显优化;骨密度、生物力学性能明显改善,差异有统计学意义(P<0.05)。结论BEMF对OVX-OP的治疗作用与TGF-β1在骨组织中的表达增强密切相关。
Objective To observe the impact of bionics pulsed electromagnetic fields on TGF-β1 in the bone tissue of ovariectomy osteoporosis rats and to explore the mechanism of the therapeuetic effect of bionics electromagnetic fields （BEMF） in postmenopausal osteoporosis. Methods Forty 6-month old female Wistar rats were randomly divided into four different groups according to body mass： ovariectomy group （OVX）, sham operation group （Sham）, E group （ Estrogen ＋ OVX） and EM group （ BEMF ＋ OVX）. All rats were subjected to bilateral ovariectomy except sham operation group. In 9 week after operation, E group rats were given estrogen 0.5mg/kg,once two weeks. EM group rats were exposed to bionics electromagnetic fields, 1h^-1· d^- 1. OVX and sham group rats were given nothing. All the treatments were kept for 10 weeks. After the treatments finished, meauring the markers as follows：the bone mineral density（BMD）（lumbar vertebrae）, the biomechanical property. Bone morphology （lumbar vertebrae） was observed by light microscope. The expression of TGF-β1 protein was detected with immunochemistry. Results Compared with OVX group, BMD and the biomechanical property significantly increased at the lumbar spine in EM group（P〈0.05）. The light microscope observation showed trabecular volume and trabecular connections significantly increased in EM group than in OVX. The imaging analyses showed that BEMF stimulation exposure significantly elevated the expression of TGF-β1 protein. Conclusion Facilitating TGF-β1 protein expression in bone tissue may be one of mechanisms of therapeutic efficacy of BEMF on postmenopausal osteoporosis.
出处
《重庆医学》
CAS
CSCD
2007年第8期726-728,共3页
Chongqing medicine
关键词
仿生脉冲电磁场
绝经后骨质疏松
转化生长因子β1
postmenopausal osteoporosis
bionics electromagnetic fields
transforming growth-β1
