摘要
应用超滤技术对经硫酸铵法浓缩的罗汉果粗酶进行分离纯化,采用截留分子量为10万、5万和1万的超滤膜对罗汉果粗酶进行分离,用酪蛋白法和考马斯亮蓝染色法分别测定酶活和蛋白质含量。研究发现酶活与蛋白质主要分布在相对分子量为10万以上和5万与1万之间,相对分子量为10万以上酶活占总酶活的28.0%,酶比活提纯了1.88倍,蛋白质量占总蛋白质量的31.7%;相对分子量为5万和1万之间的酶活占总酶活的31.2%,酶比活提纯了2.43倍,蛋白质量占总蛋白质量的33.8%。
Siraitia Grosvenorii crude enzyme of ammonium sulfate concentration were isolated and purified by Uhrafiltration technique. Then it was separated by using the ultrafihration membrane whose molecular weight cut off is 10 million, 50 000 and 10 000 separately. After that, it can use Casein and Coomassie brilliant blue method to measure the enzyme activity and protein content. The research found that the enzyme activity and protein mainly were distributed in molecular weight which was more than 10 million or between 50 000-10 000. Molecular weight of 10 million or more activity in 28.0 % of the total activity of the enzyme specific activity was 1.88 times purification, protein 31.7 % of the total protein; relative molecular weight of 50 000 and 10 000 of the total enzyme activity between 31.2 % live, enzyme specific activity was 2.43 times purification, protein 33.8 % of the total protein.
出处
《食品研究与开发》
CAS
北大核心
2012年第8期78-80,共3页
Food Research and Development
基金
广西环境工程与保护评价重点实验室项目(200912)
关键词
罗汉果粗酶
膜分离
酶活
硫酸铵法
Siraitia grosvenorii crude enzyme
membrane separation
enzyme activity
ammonium sulfate method
