摘要
目的:优选分离杭白菊中总黄酮的最佳树脂型号及工艺条件。方法:以总黄酮为指标,紫外分光光度法测定总黄酮含量,考察HPD100,DM130,D101,AB-8等不同型号大孔树脂对杭白菊中总黄酮的吸附和解吸性能,筛选最佳大孔树脂型号,并优选其分离工艺条件。结果:AB-8型大孔树脂吸附容量和解吸率均最大,分别为44.9 mg.g-1,87.9%。当供试液总黄酮质量浓度在12.6 g.L-1时,于30℃下吸附1 h,用95%乙醇进行解吸,可较好地对杭白菊中总黄酮进行吸附分离。结论:AB-8型大孔树脂适用于杭白菊中总黄酮的吸附分离。
Objective： To optimize macroporous resin for purifying total flavonoids from Chrysanthemum morifolium and its technology conditions. Method： Using the content of total fiavonoids as index which was determined by UV speetrophotometry, adsorption and desorption performance of HPD100, DM130, D101, AB-8 four macroporous resins for total flavonoids from C. morifolium were investigated, in order to screen optimum macroporous resin and optimize its separation technology conditions. Result： Adsorption capacity and desorption rate of AB-8 was the most in these resins, values were 44.9 mg· g-1 and 87.9% , respectively. Optimum separation technology was： the content of total flavonoids from sample solution 12.6 g·L-1, desorption time 1 h at 30 ℃ , 95% ethanol as eluent. Conclusion： AB-8 resin was suitable for adsorption and separation of total flavonoids from C. morifolium
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第17期53-55,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
大孔树脂
菊花
总黄酮
吸附
分离
macroporous resin
Chrysanthemum morifolium
total flavonoids
adsorption
separation
