摘要
目的研究X连锁凋亡抑制因子(XIAP)在胃癌组织中基因和蛋白水平的表达,探讨XIAP在胃癌抗凋亡特性中的作用以及与病理分级和临床分期的关系。方法采用RT-PCR检测胃癌组织及癌旁正常组织中XIAP基因的表达。采用免疫组织化学法对25例胃癌组织中XIAP蛋白表达进行检测并积分。结果胃癌组织中XIAP mRNA高表达,癌旁正常组织中XIAP mRNA低表达,两者表达率有统计学差异(P=0.000),免疫组化染色显示:XIAP蛋白主要集中在胃腺癌细胞浆中,在胃癌组织中的阳性率为84.0%(21/25),在癌旁正常胃组织中的阳性率为25.0%(6/25),两者差异有显著性(P=0.000)。XIAP蛋白表达与肿瘤病理分化程度有关(P=0.002),与临床分期无关。结论提示XIAP在胃癌中的高表达与胃癌的发生可能有关,可望作为胃癌基因治疗的一个新靶点。
Objective To observe the inhibitor of apoptosis XIAP expression of gene and protein levels in gastric cancer tissue. To discuss the anti-apoptotic characteristic of XIAP in gastric cancer and the relationship between pathological grade and clinical stage. Methods The expression of XIAP mRNA was semi-quantified by RT-PCR between gastric cancer and adjacent normal tissues, the expression of XIAP protein in 25 cases of gastric cancer was checked by Immunohistochemical method. Results The expression of XIAP mRNA in gastric cancer was higher than the expression in adjacent normal tissue（P=0.000）. Immunohistochemical method showed： XIAP protein concentrated in cytoplasm of gastric cancer, positive rate in the gastric cancer was 84.0%（21/25）, in adjacent normal tissue was 25.0%（6/25）, both differences had significantly significance （P=0.000）. The expression intensity had relationship with the degree of differentiation of tumor pathology （P=-0.002）, while had nothing to do with clinical stage.Conclusion The high expression of XIAP might be related with the occurrence of gastric cancer, XIAP could be used as a new target for gene therapy for gastric cancer.
出处
《中国医药指南》
2012年第21期443-444,共2页
Guide of China Medicine
关键词
胃癌
XIAP
细胞凋亡
凋亡抑制
Gastric caner
XIAP
Apoptosis
Inhibitor of apoptosis
