摘要
目的:研究肿瘤转移相关基因1(MTA1)与肿瘤转移抑制基因KISS-1蛋白在胃癌的表达及临床意义。方法:采用免疫组化的方法检测20例胃正常组织和82例胃癌组织中MTA1和KISS-1的表达。结果:MTA1与KISS-1蛋白在胃癌组织中阳性表达率分别为52.44%和40.24%,在正常胃组织的阳性表达率分别为5.00%和90.00%;MTA1蛋白高表达与胃癌浸润深度、组织学分级、临床分期、淋巴结转移和远隔转移的发生比较差异有统计学意义(P<0.01);KISS-1蛋白低表达与胃癌浸润深度、临床分期、淋巴结转移和远隔转移的发生比较差异有统计学意义(P<0.05);胃癌组织中MTA1与KISS-1蛋白表达呈负相关(γs=-0.364,P<0.01)。结论:MTA1蛋白水平高表达和KISS-1蛋白水平低表达与胃癌的浸润、转移密切相关,可作为诊断胃癌患者转移、复发和判断预后的参考指标,有望成为胃癌基因治疗新的靶点。
Objective：To investigate the expression and clinical significance of metastasis associated gene 1（MTA1） tumor metastasis suppressor gene KISS-1 in human gastric carcinoma. Methods：Immunnohistochemical technique was used to detect the MTAl and KISS-1 protein expression in 20 normal human gastric mucosa and 82 human gastric carcinoma samples.Results：The positive rate of MTA1 and KISS-1 protein was respectively 52.44% and 40.24% in gastric carcinoma and 5.00% and 90.00%in the normal gastric mucosa. Over-expression of MTA1 was significantly involved in the depth of invasion,grade,clinical stage,distant metastasis and lymph node metastasis（P0.01）,and the similar results were found with the low expressed KISS-1 except the grade（P0.05）.Yet,MTA1 expression was negatively related to KISS-1 in human gastric carcinoma （P0.01）. Conclusion：Over-expression of MTA1 and low KISS-1 expression were significantly involved in infiltration and metastasis of the human gastric carcinoma,and the findings suggest that MTA1 and KISS-1 may be useful markers for evaluation of tumor malignancy,predication of recurrence,metastasis and prognosis in gastric carcinoma patients,which is likely to be new target of gene therapy of gastric carcinoma.
出处
《皖南医学院学报》
CAS
2010年第4期259-262,共4页
Journal of Wannan Medical College
