摘要
利用高效液相色谱仪建立复方阿莫西林纳米乳(AMX-LH-NE)在牛奶中的残留监控高效液相色谱法(HPLC),并通过残留消除试验,研究AMX-LH-NE在乳房炎牛奶中的残留消除规律,确定其弃奶期。结果显示,阿莫西林(AMX)和盐酸左氧氟沙星(LH)分别在7.5～400μg/L和40～600μg/L线性关系良好;平均回收率为(99.72±1.46)%和(99.65±1.53)%;相对标准偏差(relative standard deviation,RSD)为1.46%和1.54%;平均保留时间为(11.59±0.21)min和(6.87±0.02)min;日内精密度RSD为1.59%和1.39%,日间精密度RSD为3.17%和3.29%。在乳房炎牛奶中,AMX和LH的残留量均随着休药时间的延长而迅速降低;AMX的弃奶期为停药后35.96h,LH的弃奶期为停药后32.94h。结果表明,所建立的HPLC方法专属性好,回收率、重复性和精密度高,可为牛奶中AMX-LH-NE的残留监控提供检测方法;利用此检测方法确定的AMX-LH-NE的弃奶期为停药后36h。
The experiment was conducted to establish the residual detection methods, research the residue elimination rule and decide the withdrawal time of compound amoxicillin and levofloxacin hydrochloride nanoemulsion （AMX- LH-NE） in milk from cows with mastitis disease. Two different high performance liquid chromatography （HPLC） analytical methods for residual concentration monitoring of amoxicillin（AMX） and levofloxacin hydrochloride （LH） were established, respectively. Then the withdrawal time was decided by the residue elimination trial. The results showed that two good linearity were obtained by AMX in the range of 7.5-400 μg/L and by LH in the range of 40- 600 μg/L, respectively, and the average recovery, RSD （relative standard deviation）, the average retention time, RSD of the with-in-day precision,RSD of the day-to-day precision of AMX and LH were （99.72±1.46）% and （99.65±1.53） %,1.46% and 1.54% ,（11.59±0.21） rain and （6.87±0.02） min,1.59%0 and 1.39,3.17% and 3.29%, respectively. The results of the residue elimination experiment indicated that residual AMX and LH quickly cut down with prolongation of withdrawal time in milk respectively. The withdrawal time of AMX was 35.96 h,while that of LH was 32.94 h. The two HPLC analytical methods possessed the good specificity,high recovery rate, repetitiveness and precision,it could offer the detection approach for residue monitoring of AMX LH NE in milk. 36 h of the clini cal withdrawal time of AMX-LH-NE was recommended when it is used to treat bovine clinical mastitis in the lacta tion period.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第8期1212-1216,共5页
Chinese Journal of Veterinary Science
基金
国家科技支撑计划奶业发展重大关键技术研究与示范资助项目(2006BAD04A11)
关键词
纳米乳
阿莫西林
盐酸左氧氟沙星
牛奶
残留量
弃奶期
nanoemulsion
amoxicillin
levofloxacin hydrochloride
milk
residue level
withdrawal time
