摘要
建立了铽(Tb3+)-左氧氟沙星络合物荧光增敏法测定牛奶中左氧氟沙星的含量。左氧氟沙星与Tb3+结合后,543nm处出现络合物的强荧光峰。最终确定了测定条件:200倍的Tb3+,pH6.0醋酸-醋酸铵缓冲溶液,激发波长和发射波长为291和543nm。左氧氟沙星在0.01～2μg/ml范围内,浓度与荧光强度成良好的线性关系,检测限为2.5ng/ml。
Terbium （Tb^3＋） -levofloxacin complex fluorescence enhancement method was established for determination of levofloxacin in milk. The fluorescence peak of terbium （Tb^3＋） -levofloxacin complex was increased on 543nm. The determination condition was： 200 times Tb^3＋, HOAc-NH4OAc buffer solution （pH 6.0）, the excitation and emission wavelength was 291nm and 543nm. The calibration curve was liner in the concentration range of 0.01 - 2μg/ml. The detection limit was 2.5ng/ml.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2008年第1期42-45,共4页
Chinese Journal of Pharmaceuticals
基金
河南省杰出人才创新基金(421002300)
关键词
左氧氟沙星
残留
铽
荧光
牛奶
测定
levofloxacin
residue
terbium
fluorescence
milk
determination
