摘要
[目的]考察柴胡提取物中总皂苷的最佳测定方法。[方法]以总皂苷吸光度为评价指标,采用单因素试验优选柴胡总皂苷的测定方法。[结果]总皂苷的最佳测定方法为:供试品溶液0.2 ml,加入0.4%对二甲氨基苯甲醛乙醇溶液0.1 ml在70℃反应10 min,放冷后至室温后,加入4 ml磷酸在50℃下反应20 min。[结论]该方法稳定可行,适用于柴胡总皂苷的含量测定。
[ Objective] To set up an optimal method for the determination of total saponins from RADIX BUPLEUR1 extracts. [ MethodJ The determination method was optimized by single factor test according to the absorbance of total saponin. [ Result] The best determination method of total saponin was ： 0.2 ml test solution was added with 0.1 ml 0.4% p-dimethylaminobenzaldchyde ethanol solution under 70 ℃ thermostat- ic waterbath for 10 minutes, after cooling off, 4 ml phosphoric acid was added under 50 ℃ thermostatic waterbath for 20 minutes. [ Conclusion ] The method is table and practical, and it is applicable to the determination of total saponin from RADXI BUPLEURI extracts.
出处
《安徽农业科学》
CAS
2012年第24期11998-11999,12002,共3页
Journal of Anhui Agricultural Sciences
基金
西南科技大学-四川德培源中药科技开发有限公司校企联合基金目(11zh0093)
