摘要
为对骨膦的药效学进行综合评价，取8月龄雌性Wistar大鼠44只，随机分为4组，每组11只。骨膦组大鼠切除双侧卵巢后，皮下注射骨膦12周，与单纯卵巢切除组（OVX组）、假手术组（SHAM组）及口服尼尔雌醇组（E3组）比较。用骨生物力学、骨密度测量和骨形态计量学等方法对骨膦的药效学进行综合评价。结果：与SHAM组比较，OVX组骨灰重、骨干重、骨钙含量和骨密度、抗弯力、骨小梁体积密度及宽度均明显降低，而成骨细胞指数和破骨细胞指数、尿羟脯氨酸则明显增高。骨膦和E3能使上述指标显著改善，接近正常水平。由此表明骨膦和E3能有效防止去势雌鼠骨量的丢失。
To evaluate the anti-osteoporosis effect of clodronate in ovariectomized rats, 44 female Wistar rats,aged 8 months, were divided randomly into 4 groups: the SHAM group (subjected to sham operation) ; the OVX group (subjected to bilateral ovariectomy); the E3 group (subjected to bilateral ovariectomy and treated after surgery with nilestriol lmg/kg BW by gavage once a week for 12 weeks), and the clodronate group (subjected to bilateral ovariectorny and treated after surgery with clodronate 5mg/kg BW by subcutaneous injection twice a week for 12 weeks). All the animals were sacrified 12 weeks after the operation. The blood and urine samples were col1ected for determination of biochemical indices. The humeri and tibiae were prepared for determination of net weight, ash weight, calcium and phosphorus content, bone density, as we1l as bone histopathology and histomorphometry. The left femora were processed for bone anti-curve power. The results showed that as compared with the SHAM group, the net weight, ash weight, calcium content, density and anti-curve power of bones, te trabecular bone volume and thickness were all significantly reduced in the OVS group, while its osteoblast index, osteoclast index and urine hydroxyproline content were obviously close to normal levels. The data indicates that both clodronate and E3 can effectively prevent bone loss in ovariectomized rats.
出处
《华西医科大学学报》
CAS
CSCD
2000年第2期226-229,共4页
Journal of West China University of Medical Sciences
关键词
骨膦
骨质疏松
骨生物力学
卵巢切除术
大鼠
Clodronate Ovariectomy Osteoporosis Bone biodynamics Bone histomorphometry
