摘要
目的探讨以壳聚糖为载体、采用乳化交联法制备的bFGF壳聚糖微球体外释放特性,为下一步实验奠定基础。方法应用0.6%三聚磷酸钠溶液作为交联剂,1.5%壳聚糖溶液作为载体,采用乳化交联法制备bFGF壳聚糖微球。激光粒度及Zeta电位分析仪检测微球粒径分布,扫描电镜观察形态;ELISA法计算bFGF壳聚糖微球载药量、包封率及体外释药规律。结果 bFGF壳聚糖微球粒径为20.312～24.152μm;扫描电镜观察显示微球表面光滑圆整,无明显孔隙,分布均匀,分散性好。载药量和包封率分别为(7.57±0.34)mg/g及95.14%±1.58%。bFGF壳聚糖微球可持续体外释放bFGF 24 d;bFGF浓度随时间延长逐渐升高,第24天达(820.45±21.34)ng/mL;微球体外释药具有突释效应,突释率为18.08%,24 d累计释放率为82.05%。结论乳化交联法制备bFGF壳聚糖微球操作简便,微球表面光滑、分布均匀,分散性好,载药量和包封率均较高,体外释药较稳定且释放率较高,是一种较理想的制备bFGF壳聚糖微球的方法。
Objective To study the release properties of basic fibroblast growth factor （bFGF） chitosan microspheres prepared by cross-linking-emulsion method using chitosan as a carrier material so as to lay a foundation for further study. Methods Using 0.6% sodium tripolyphosphate solution as a crosslinking agent and 1.5% solution of chitosan as a carrier material, bFGF chitosan microspheres were prepared by cross-linking-emulsion method. Laser particle size analyzer and Zeta electric potential analyzer were used to measure the particle diameter distribution, scanning electronic microscope to observe the morphology, and ELISA to determine the drug loading, the encapsulation rate, and the drug release properties. Results The particle size ofbFGF chitosan microspheres ranged 20.312-24.152 μm. The microspheres were round with a smooth surface and uniform distribution, and it had no apparent porosity. The drug loading and encapsulation rate of microspheres were （7.57 ±0.34） mg/g and 95.14% ±1.58%, respectively. The bFGF chitosan microspheres could continuously release bFGF for 24 days; the bFGF level increased gradually with time and reached （820.45± 21.34） ng/mL at 24 days; and the microspheres had a burst effect, and the burst rate was 18.08%, and the accumulative release rate of the microspheres reached 82.05% during 24 days. Conclusion It is easy-to-operate to prepare the bFGF chitosan microspheres with the cross-linking- emulsion method. The bFGF chitosan microspheres have smooth surface, uniform distribution, and no apparent porosity.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2012年第8期989-992,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
四川省科技厅课题资助项目(05SG022-037)~~
关键词
BFGF
壳聚糖微球
乳化交联法
壳聚糖
Basic fibroblast growth factor Chitosan microsphere Cross-linking-emulsionmethod Chitosan
