摘要
背景与目的:血管内皮生长因子(vascular endothelial growth factor,VEGF)不但能诱导肿瘤血管生成,而且直接参与肿瘤的免疫抑制,诱导肿瘤的免疫逃逸。本文旨在探讨VEGF与树突状细胞(dendritic cell,DC)的成熟及调节性T细胞(regulatory T cell,Treg)在小细胞肺癌组织微环境中的关系及其与肿瘤患者临床特征的相关性。方法:收集天津医科大学附属肿瘤医院2000年1月—2010年9月期间手术的13例小细胞肺癌患者术后癌组织标本及癌旁正常组织标本,以及61例小细胞肺癌患者术后癌组织标本,采用免疫组化方法,检测石蜡切片中VEGF、CD1a、CD83和Foxp3的表达情况,并用SPSS 17.0统计软件分析它们之间的相关性及其与患者临床特征的关系。结果:在13例小细胞肺癌患者中,VEGF在癌组织中的表达明显高于癌旁正常组织(P=0.000)、表达CD1a的DC数量在癌组织5.49±3.55明显高于癌旁正常组织1.94±0.86(P=0.007)、表达CD83的DC数量在肺癌组织0.72±0.77明显低于癌旁正常组织3.15±2.32(P=0.002)、表达Foxp3的T细胞阳性比例在癌组织中(5.88±4.21)%显著高于癌旁正常组织(0.031±0.075)%(P=0.005)。61例小细胞肺癌患者癌组织中VEGF表达的阳性率为81.97%(50/61),CD1a+DC在VEGF阴性、阳性表达中差异无统计学意义(P>0.05),CD83+DC在VEGF阴性、阳性表达中差异有统计学意义(P=0.000),Foxp3+Treg细胞阳性比例在VEGF阴性、阳性表达中差异无统计学意义(P>0.05)。CD1a+DC与Foxp3+Treg细胞阳性比例之间呈正相关(r=0.373,P=0.007)。CD1a、Foxp3表达在肿瘤患者不同年龄、肿瘤大小、肿瘤类型、临床分期、淋巴结转移情况之间差异无统计学意义。CD83表达在不同肿瘤大小(直径<3 cm,直径>3 cm)患者之间差异有统计学意义(P<0.05)。结论:在小细胞肺癌中,VEGF、成熟DC、不成熟DC、Treg细胞在癌组织与癌旁正常组织的表达有差异。VEGF可抑制DC的成熟,而不成熟的DC数量与Treg细胞数量呈�
Background and purpose： Vascular enctotlaellal growm factor not omy can induce tumor angiogenesis but also can participate directly in the tumor immunosuppression, inducing tumor immune to escape. This study aimed to investigate the relationship between vascular endothelial growth factor, dendritic cells（DC） and regulatory T cells（Treg） in human small cell lung cancer microenvironment, and the relationship between them and clinical characteristic of small cell lung cancer. Methods： tissue and surrounding normal tissues, 61 cases of small Thirteen cases of small cell lung cancer paraffin-embedded cell lung cancer paraffin-embedded tissues, were collected, and immunohistochemistry was used to detect the expression of VEGF, CDla, CD83 and Foxp3. Then the relationship between them were analyzed. Results： Positive expression of VEGF in small cell lung cancer tissue was detected in 50 of 61 （81.97%） cases. Expression of CDla＋DC in small cell lung cancer tissue （5.49±3.55） was higher than that in surrounding normal tissue （1.94±0.86, P=-0.007）. Expression of CD83＋DC in small cell lung cancer tissue （0.72±0.77）was lower than that in surrounding normal tissue （3.15±2.32, P=0.002） . The percentage of Foxp3 positive T cell in small cell lung cancer tissue [（5.88±4.21）%] was higher than that in surrounding normal tissue [（0.031±0.075%, P=0.005）]. CDla positive cells was not statistically significant between the group of VEGF positive and the one of VEGF negative （P〉0.05）. CD83 positive cells was statistically significant between the group of VEGF positive and the one of VEGF negative （P〈0.05）. There was a positive correlation between the expression of CD1 a＋DC and percent of Foxp3＋Tregs in small cell lung cancer （r=0.373, P=-0.007）. Positive expressions of CDla, Foxp3 were not related to patient＇s age, tumor size, clinical stage and lymphatic metastasis. Positive expression of CD83 was related to tumor size, but not related to patient＇s age, clinical
出处
《中国癌症杂志》
CAS
CSCD
北大核心
2012年第7期517-521,共5页
China Oncology
关键词
小细胞肺癌
血管内皮生长因子
树突状细胞
TREG细胞
Small cell lung cancer
Vascular endothelial growth factor
Dendritic cell
Regulatory T cell
