摘要
从美丽海绵分离提取了2-去氧-1-氢-1,2,4,三唑、环(脯氨酸-甘氨酸)、尿嘧啶、环(脯氨酸-丙氨酸)、6-氨基嘌呤、4-(1-苯乙基)苯酚、1-氢-1,2,4,三唑和胆甾-5-烯-3β,7α-二醇等8种化合物,并研究了其对网纹藤壶金星幼虫的影响,其中6-氨基嘌呤(7.1μg/cm2)和4-(1-苯乙基)苯酚(5.9μg/cm2)对金星幼虫具有明显的毒杀作用。美丽海绵可能通过这些活性化合物的协同作用来防止它种生物附着污损。
Abstract： Around the woad, marine fouling can have serious impacts on human activities and other organisms. To survive in the highly competitive arena of the marine environment, many organisms have developed unique protection mechanisms against fouling, including tolerance, avoidance, low surface energy and the secretion of natural compounds. Some species of sponge and coral are rarely epiphytized because of the production of secondary metabolites against fouling. Unlike common man-made antifouling compounds, these natural chemicals are environmentally friendly in marine ecological systems. The majority of marine fouling organisms are algae, coelenterates, polychaetes, bivalves, bryozoans and bamaeles. Of them, the acorn barnacle Balanus reticulatus is one of the most important dominant species in the fouling communities of tropical and subtropical waters, particularly in the East and South China Sea. Thus is can be considered as an appropriate test representative for antifouling bioassays. Barnacle nauplii were obtained by dissecting freshly collected adult barnacles from the acuacuhure facilities at Daya Bay, Shenzhen, China. Bamaele larvae were reared in darkness, at 30°C and on a diet of the green algae Platymonas subcordiformis. Seawater was changed and more algae added as necessary. Cyprid larvae developed after around 5 days and were collected and stored at 4°C for subsequent use. Sponges of the genus Callyspongia are widely distributed in the southern waters of China （ e. g. coastal water of Fujian, Guangdong, Guangxi and Hainan Provinces ）. They are well known as sources of biologically active natural products, including polyacetylenes, peptides, alkaloids, fatty acids, polyketides, and sterols. Some cf these compounds possess antimicrobial, anti-tumor, cytotoxicic, and HIV reverse transcriptase inhibition properties. Freshly collected samples of Callyspongia were minced and extracts taken by washing with dilute ethanol three times.The aqueous ethanol extracts were concentrated under vacuum.
出处
《生态学报》
CAS
CSCD
北大核心
2012年第13期4285-4290,共6页
Acta Ecologica Sinica
基金
国家自然科学基金(31100260
41176102)
广东省海洋渔业科技推广专项重点项目(A201101F03)
关键词
海绵
提取物
藤壶
幼虫
sponge
compounds
barnacle
larvae
