摘要
目的:研究槐定碱对心力衰竭大鼠心肌细胞钙诱导钙释放的影响。方法:结扎大鼠冠状动脉左前降支制备心力衰竭模型,随机分为假手术组、心力衰竭组、槐定碱5 mg/kg、10 mg/kg和20 mg/kg剂量组。4周后酶解法分离各组大鼠心室肌细胞,采用膜片钳和激光扫描共聚焦显微镜同步实时记录系统记录心肌细胞的L-型钙电流(ICa.L)及胞浆内的钙瞬变。结果:心力衰竭组大鼠心肌细胞的ICa.L、钙火花发生率及钙瞬变峰值明显低于假手术组;槐定碱中剂量和高剂量组大鼠心肌细胞的ICa.L、钙火花发生率及钙瞬变峰值明显高于心力衰竭组;槐定碱低剂量组与心力衰竭组相比差异不显著。结论:一定浓度的槐定碱对改善心力衰竭大鼠心肌细胞钙诱导钙释放功能具有重要的作用。
Objective： To explore the effect of sophoridine on Ca^2＋-induced Ca^2＋ release in cardiomyoeyte of the heart failure rat. Methods ： The experimental heart failure （HF） was induced by permanent ligation on the left anterior descending coronary artery in SD- rat, these rats were randomly divided into five groups： sham operated rats; heart failure rats; low dose sophoridine treated group （5 mg/kg） ; middle dose sophoridine treated group （10 mg/kg） , and high dose sophoridine treated group （20 mg/kg）. Four weeks later, the single cardiomyocyte of the above rat hearts was isolated by an enzymatic dissociation method. Patch-clamp and laser scanning confocal microscope synchronous recording system software were used to record transmembrane Ca^2＋ currents （Ica. L ） and Ca^2＋ transients in cytoplasm. Results： The amplitude of Ica. L was reduced in HF cardiomyocytes compared with the sham, and moreover, the amplitude of Ca^2＋ transients and spatial averages of Ca^2＋. transients were also dramatically reduced. The amplitude of Ica. L was enhanced in middle and high dose sophoridine treated group, and the amplitude of Ca^2＋ transients was enhanced as well. In addition, there was a significant increase in spatial averages of Ca^2＋ transients in middle and high dose sophoridine treated group and there was no significant difference in the low dose sophoridine treated group. Conclusion： Sophoridine of certain concentration can improve the function of Ca^2＋ -induced Ca^2＋ release in cardiomyocyte of experimental rats with heart failure.
出处
《电子显微学报》
CAS
CSCD
2012年第3期235-242,共8页
Journal of Chinese Electron Microscopy Society
基金
军队医药卫生"十一.五"科研项目(06MB202)
关键词
槐定碱
心肌细胞
心力衰竭
钙诱导钙释放
L-型钙电流
激光扫描共聚焦显微镜
sophoridine
cardiomyocyte
heart failure
Ca^2＋ -induced Ca^2＋ release
L-type calcium current
laser scanning confocal microscope
