摘要
目的分析血管紧张素转换酶(ACE)基因多态性与原发性高血压(EH)的关系。方法选择煤矿井下接尘工人为研究对象(EH组和对照组各206人),进行职业流行病学调查,并收集研究对象空腹静脉血5 ml,酚氯仿法提取基因组DNA,采用聚合酶链反应(PCR)方法检测ACE基因多态性。比较两组间的基因型、等位基因频率及倒班分布的差异。结果 EH组和对照组倒班作业工人所占的比例分别为42.23%和29.61%,组间分布比较差异有统计学意义(χ2=7.13,P<0.05),EH发生的危险增加了1.74倍。ACE基因II、ID、DD基因型和等位基因I、D的分布,差异无统计学意义(P>0.05),提示ACE基因多态性与EH无直接关系;但倒班与I和D等位基因联合作用的OR值分别为1.86(OR95%CI:1.32～2.63)和1.72(OR95%CI:1.09～2.71),相乘模型交互作用产生的OReg值为0.79,提示倒班与ACE基因I/D等位基因之间存在负交互作用(似然比检验P<0.05)。结论倒班可能是EH的危险因素且可能与ACE基因插入/缺失序列等位基因存在交互作用。
Objective To explore the relationship between ACE gene polymorphism and essential hypertension in underground coal workers in China. Methods A case-control study was conducted in 412 coal workers （206 essential hypertension workers and 206 healthy controls）. 5 ml of fasting vein blood samples were taken for ACE L/D polymorphism detection with PCR and compare the differences in genetype allele frequency and shift work between two groups. Results The results showed that the proportion of shift-work in EH cases （42. 23% ） was significantly higher than that in controls （29. 61% ）, and the odds ratio （OR） for EH was also 1.74 times increased comparing with the controls, There was no significant difference in frequency of ACE （I/D） genetypes between EH group and control group, neither the frequency of I and D allele. But there was a close correlation between shift work and allele of ACE （L/D） gene. Conclusion Shift work may be the risk factor of EH in coal workers which had a negative interaction with allele of ACE （L/D） gene,
出处
《中国工业医学杂志》
CAS
北大核心
2012年第3期197-199,共3页
Chinese Journal of Industrial Medicine
