摘要
目的探讨β2肾上腺素能受体(β2-AR)Arg16Gly基因多态性及与职业精神紧张的交互作用对原发性高血压(EH)发生的影响。方法以职业紧张高暴露的男性行车人群为基础的1∶1病例对照研究(95对),调查职业紧张、紧张反应、焦虑、A型行为等职业精神紧张及高血压的常见危险因素,应用PCR-RFLP技术检测β2-ARArg16Gly基因多态性。采用似然比检验判断因素效应之间是否有相乘模型OR交互=OR11/(OR01×OR10)的交互作用。结果病例组和对照组的β2-AR16位点基因Arg/Arg、Arg/Gly、Gly/Gly频率分布有差异,具有统计学意义(P<0.05),在调整BMI等影响因素后,携带Gly/Gly的个体患EH的OR值为2.759(95%CI:1.200～6.347);携带突变型Arg16Gly基因与紧张反应、特质焦虑对原发性高血压影响的交互作用符合相乘模型,当携带突变型Arg16Gly基因与高紧张反应、特质焦虑同时存在时,其患EH的风险分别增加至12.797(95%CI:3.379～48.461),40.862(95%CI:1.403～119.057)。结论β2-AR基因Arg16Gly多态性很可能是职业紧张高暴露男性行车人群EH发生的重要易感因素;携带突变型Arg16Gly基因的个体,暴露于紧张反应和特质焦虑时,其原发性高血压发生的危险性显著增加。
Objectlve To explore the role of the interaction between β2-adrenergic receptor（β2-AR）gene Arg16Gly polymorphism and occupational mental stress in EH. Methods Using 1 ： 1 case-control study, we select 95 couple people as the study objects from the high-exposure occupational stress males who work on the train. Measuring occupational stress, stress reaction, anxiety, type A behavior and other occupational mental stress by using proper questionnaire, and applying PCR-RFLP technology to detect adrenergic receptor（β2-AR） Arg16Gly Polymorphism. Using likelihood ratio test to judge whether it exist the interaction between the potencies and fits the multiplicative model： OR = OR11/（ OR01 × OR10 ）. Results The distribution of Arg/Arg, Arg/Gly, Gly/Gly in essential hypertension cases and controls are significant difference （ P 〈 0.05 ）, Gly/Gly had a increased risk of hypertension 2.759 （95 % CI： 1. 200 - 6. 347） compared to Arg/Arg upon controling BMI and other factors. The interaction among Arg16Gly（A/G ＋ G/G） gene,occupational stress reaction and anxiety is fit the muhiplicative model. The results showed that when Arg16Gly（ A/G ＋ G/G） genotype interact with strain, the risk of EH will add to 12. 797（95 % CI：3. 379 - 48.461 ）. When it interact with anxiety, the risk of EH will add to 40. 862（95 % CI：1.403 - 119.057）. Conclusion Arg16Gly polymorphism of β2-AR might be the crucial risk factor for EH among high-exposure occupational stress males. Individual who has genotype Gly/Gly, while exposure of strain and T- Anxiety is much easier to get EH.
出处
《卫生研究》
CAS
CSCD
北大核心
2009年第4期445-448,共4页
Journal of Hygiene Research
基金
福建省医学创新课题(No.2007-CX-8)
关键词
原发性高血压
Β2肾上腺素能受体
单核苷酸多态性
职业精神紧张
基因-环境交互作用
essential hypertension, beta-2adrenergic receptor, single nucleotide polymorphism, occupational mental stress, environment-gene interaction
