摘要
[背景]原发性高血压(essential hypertension,EH)的发病由多种遗传和环境因素共同决定。在遗传因素中,CYP11B2基因-344T/C多态性的研究较多,高温作为职业危险因素,二者有可能交互作用于EH。[目的]探讨CYP11B2基因-344T/C多态性和高温作业的交互作用对钢铁工人EH易感性的影响。[方法]选择793名钢铁企业男性工人进行病例对照研究,包括EH组284例和对照组509例。对研究对象开展职业流行病学调查,采集静脉血。检测工作场所高温与噪声暴露情况。采用聚合酶链反应-限制性片段长度多态性检测工人CYP11B2基因-344T/C的多态性。采用多因素logistic回归和叉生分析方法分析CYP11B2基因-344T/C多态性与高温作业的相乘及相加交互作用对男性钢铁工人EH易感性的影响。[结果]等位基因分析中,携带C等位基因工人患EH的危险性是携带T等位基因者的1.39倍(95%CI:1.09~1.77)。多因素logistic回归显示,高温作业者患EH的危险性是非高温作业者的1.43倍(95%CI:1.03~2.00),携带CC基因型工人患EH的危险性是TT型的2.48倍(95%CI:1.38~4.47)。高温和CYP11B2基因-344T/C多态性未显示出对于EH的相加交互作用(U=-0.258,P> 0.05)与相乘交互作用(CC基因型OR=2.77,95%CI:0.97~7.88)。[结论]CYP11B2基因-344T/C多态性中CC基因型可能是患EH的易感基因,在与EH的分析中,未发现其基因多态性与高温作业存在相加或相乘的交互作用。
[Background] The onset of essential hypertension (EH) is determined by a combination of genetic and environmental factors. Among the genetic factors, CYP11B2 gene-344T/C polymorphism is frequently studied. High temperature as an occupational risk factor and the polymorphism may have an interaction effect on EH.[Objective] This study investigates the effect of the interaction between CYP11B2 gene-344T/C polymorphism and high temperature exposure on the susceptibility of steel workers to EH.[Methods] A case-control study was conducted among 793 male workers in an iron and steel enterprises, including 284 EH patients and 509 controls. An occupational epidemiological survey was conducted and venous blood samples were collected. High temperature and noise exposure in workplace were detected. CYP11B2 gene-344T/C polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism analysis. Multiple logistic regression and fork analysis were used to analyze the additive and multiplying effects of CYP11B2 gene-344T/C polymorphism and high temperature operation on the susceptibility of male steel workers to EH.[Results] In the allelic analysis, the risk of developing EH in C allele carriers was 1.39 times that in T allele carriers (95%CI: 1.09-1.77). The multiple logistic regression results showed that the risk of developing EH in workers with high temperature operation was 1.43 times that in workers without (95%CI: 1.03-2.00), and the risk in workers carrying CC genotype was 2.48 times that in workers carrying TT genotype (95%CI: 1.38-4.47). High temperature and CYP11B2 gene-344T/C polymorphism did not show additive interaction (U=-0.258, P > 0.05) or multiplication interaction (CC genotype OR=2.77, 95%CI: 0.97-7.88) for EH.[Conclusion] The CC genotype of CYP11B2 gene-344T/C polymorphism is associated with elevated EH susceptibility. No additive or multiplication interaction between its gene polymorphism and high temperature operation is found.
作者
王金
王颖
李培帅
秦萌
蒋守芳
佟俊旺
WANG Jin;WANG Ying;LI Pei-shuai;QIN Meng;JIANG Shou-fang;TONG Jun-wang(School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063210, China;Department of Endocrinology, Affiliated Hospital of North China University of Science and Technology, Tangshan, Hebei 063000, China)
出处
《环境与职业医学》
CAS
CSCD
北大核心
2019年第9期831-835,共5页
Journal of Environmental and Occupational Medicine
基金
河北省卫生和计划生育委员会项目(20160216)
关键词
高血压
高温作业
基因多态性
交互作用
hypertension
high temperature operation
gene polymorphism
interaction
