摘要
目的建立应用于手足口病毒分离和对手足口EV71病毒抑制药物筛选的微量细胞培养方法。方法比较微量细胞培养结合real-timePCR和直接应用real-timePCR两种方法检测手足口病毒的差别,并应用微量细胞培养采用细胞病变效应法和MTT分析法,观察利巴韦林对EV71病毒的抑制作用。结果经微量细胞培养后EV71、CA16阳性检出率为90%和80%,但PE阴性样本20%检测为EV71阳性;利巴韦林浓度为0.4、0.2、0.1mg/mL时对EV71病毒有抑制作用,抑制率分别为30.28%、28.09%和29.16%。结论微量细胞培养在手足口病毒分离检测和对其抑制作用药物筛选研究中具有可行性,且其具有操作简便、省时省力等优点。
Objective To construct a trace cell culture method for isolating the hand-foot-and-mouth virus and screening drugs to inhibit the EV71 virus. Methods The differences of EV71 virus detection using a combination of trace cell culture and real-time PCR or single real-time PCR were studied, and inhibition effects of ribavirin against EVT1 were investigated through the trace cell culture using cytopathic effect and MTT analysis. Results Through the trace cell culture, EV71 and CA-16 positive detection ratio values were 90% and 80%, respectively; and the EV71-positive value was 20% in PE negative samples. With the ribavirin concentration of 0.4,0.2,0.1 mg/mL,the inhibition ratio values against EVT1 virus were 30.28%, 28.09% and 29.61%, respectively. Conclusion The trace cell culture method is feasible for the isolating and detecting the hand-foot-and-mouth virus, and also the screening of inhibition drugs. Meanwhile, this method is more simple, convenient and time-saving.
出处
《中国医药科学》
2012年第11期14-15,29,共3页
China Medicine And Pharmacy
基金
黑龙江省哈尔滨市科技创新人才研究专项资金项目(2009RFQQS007)
关键词
微量细胞培养
手足口
病毒分离
药物筛选
Trace cell cuhure
Hand-foot-and-mouth disease
Virus isolation
Drug screening
