摘要
介绍肠道病毒(EV)诊断与分类的主要实验室检测方法。传统的病毒培养与血清中和试验方法是金标准,但具有费时、繁琐,且敏感性不高等局限性,而血清型别多样性又限制其血清学检测方法的应用。分子生物学检测技术的发展,为EV的诊断与分类提供了快速、准确的方法,而且能够提供其它方法所无法提供的生物信息。
To review the primary methods for identification and classification of enteroviruses. The conventional methods, virus isolation and neutralization with serotype-specific antisera, have been regarded as golden standard for enteroviruses identification and typing, but there are several drawbacks such as time-consuming, labor-intensive and low sensitivity. And serological diagnosis of enterovirus infections is complicated by the large number of serotype. And a rapid and definitive results are most likely to be achieved by molecular methods, which provide additional bio-informations.
出处
《中国计划免疫》
2006年第3期226-229,共4页
Chinese Journal of Vaccines and Immunization
关键词
肠道病毒
诊断
分类
Enteroviruses
Diagnosis
Classification
