摘要
目的:通过紫外-可见分光光度法与高效液相色谱法对萘酚喹的含量测定进行对比,为建立萘酚喹的定量方法提供依据。方法:UV法以0.05 mol.L-1盐酸溶解样品,在341 nm处测定;HPLC色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm),流动相乙腈-0.25%二乙胺溶液(pH 2.5)(23∶77),流速0.8 mL.min-1,检测波长342 nm。结果:UV法研究表明萘酚喹在5.1～15.3 mg.L-1线性关系良好(r=0.999 9),平均回收率为100.0%,RSD 0.3%(n=9);HPLC法研究表明萘酚喹在0.16～0.8μg与峰面积呈良好的线性关系(r=0.999 9),平均回收率为99.9%,RSD 0.2%(n=9)。UV,HPLC与非水溶液滴定法对比测定结果无明显差异。结论:UV与HPLC均可用于萘酚喹的含量测定。
Objective： To study the determination methods for naphthoquine by Ultraviolet Spectrophotometry （UV） and High Performance Liquid Chromatography （HPLC）, to provide data for the quantitative determination of naphthoquine. Method： To UV, 0.05 mol· L-1 hydrochloric acid was used to dissolve the sample then test at wavelength 341 nm. HPLC was achieved on a Diamonsil C18 column （4.6 mm × 250 mm, 5 μm） utilizing a mobile phase which consisted of aeetonitrile and 0.25% diethylamine （pH 2.5） （23： 77）. The flow rate was 0.8 mL· min-1, and the detection wavelength was at 342 nm. Result： To UV, naphthoquine has a good linearity at the range of 5.1-15.3 mg · L-1 （r =0. 999 9） , and the average recovery is 100.0% with RSD 0.3% （n =9）. As to HPLC, naphthoquine has a good linearity at the range of 0. 16-0.8 μg （r=0. 999 9） , and the average recovery was 99.9% with RSD 0.2% （n =9）. The assay results among UV, HPLC and non-aqueous titration showed little difference. Conclusion： Both methods of UV and HPLC are suitable for the determination of naphthoquine.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第12期103-105,共3页
Chinese Journal of Experimental Traditional Medical Formulae
