摘要
目的:使用非水溶液滴定法对萘酚喹的含量测定方法进行研究,为确定萘酚喹的定量方法提供依据。方法:以冰醋酸为溶剂溶解样品,α-萘酚苯甲醇为指示剂,用0.1 mol.L-1高氯酸滴定液进行滴定,以自动电位滴定法同时验证滴定终点;对测定的精密度、中间精密度、稳定性、回收率等进行方法学研究,并与紫外-可见分光光度法(UV)、高效液相色谱法(HPLC)进行对比测定。结果:非水溶液滴定法滴定终点突跃明显(蓝色→黄绿色),自动电位滴定法证明滴定的终点准确无误;平均回收率为100.0%,RSD 0.1%(n=6),与UV,HPLC对比测定的结果无明显差异。结论:非水溶液滴定法测定抗疟药萘酚喹的含量,方法准确、重复性好、操作简单,适用于萘酚喹的含量测定。
Objective：To establish a method for the assay of naphthoquine by non-aqueous titration,so as to provide more data for the quantitative determination of naphthoquine.Method： The non-aqueous titration was carried out by using glacial acetic acid as solvent,α-naphthalphenol benzyl alcohol as indicator,and titrated with perchloric acid volumetric solution at a concentration of 0.1 mol·L-1.Meanwhile,the end point of titration was validated by auto-potentiometric titration,and study the precision,intermediate–precision,stability and recovery of the method were also studied,and compared the method with two other methods,UV and HPLC.Result： End point of the non-aqueous titration was obvious（blue→greenyellow）,which was also identified simultaneously by Auto-potentiometric titration.The average recovery was 100.0% with RSD 0.1%（n=6）.The results of comparison showed little difference among the three kinds of methods.Conclusion： The method of non-aqueous titration has a good accuracy,repetitiveness and easy to carry out,which is suitable for the assay of naphthoquine.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第5期104-106,共3页
Chinese Journal of Experimental Traditional Medical Formulae
