摘要
目的 探索生长抑素受体 5种亚型 (SSTR1～ 5 )在肺癌中的分布与表达特性。方法 以 [α 35S]dATP标记SSTR 5种亚型的寡核苷酸为探针 ,运用原位杂交技术 ,检测 2 1例不同病理类型肺癌组织中SSTR1～ 5mRNA的分布模式 ,并用Leica图像分析仪对其表达密度作半定量分析。结果 ①SSTR1～ 5在肺癌中的分布 :在小细胞肺癌中以SSTR2为主 ,而在肺腺癌、肺鳞癌 2种非小细胞肺癌中以SSTR1为主 ;②SSTR1～ 5在肺癌中的半定量分析 :非小细胞肺癌类受体含量高于小细胞肺癌 (P<0 0 1)。结论 肺癌中SSTR各亚型的分布模式和表达密度具有病理组织类型间的异质性 ;小细胞肺癌和非小细胞肺癌都有SSTR的表达 。
Objective To investigate the characteristics of expression and distribution of 5 subtypes of somatostatin receptors (SSTR1～5) in lung cancer Methods With [α 35 S]dATP labeled oligonucleotides of the 5 SSTR subtypes as probes, using in situ hybridization, patterns of mRNA expression were detected in lung cancer tissue sections of 21 cases which fell in varied pathologic types Additionally, Leica Q 500 image analyzing device was employed to semi quantitatively analyze density of the expression Results Patterns of SSTR1～5 expression in lung cancer were as follows: SSTR2 expression was dominant in small cell lung cancer (SCLC) while in non small cell lung cancer (NSCLC) such as adenous and squamous, SSTR1 expression was stronger than that of the other 4 subtypes In density of SSTR1～5 expression in lung cancer, NSCLC was higher than SCLC (P<0 01) Conclusions Even though patterns and density of expression of SSTR subtypes in the lung cancer showed heterogeneity in different histopathologic types, as in SCLC and in NSCLC Therefore, it has positive prospects for somatostatin analog oriented agents to be used in treatment of both types of the lung cancers
出处
《中华核医学杂志》
CAS
CSCD
北大核心
2000年第1期16-18,共3页
Chinese Journal of Nuclear Medicine
基金
国家青年自然科学基金!资助项目 ( 3 95 0 0 0 3 5 )
