摘要
目的:采用直接标记法将99mTc标记depreotide,筛选depreotide的最佳标记条件,建立一种简便可靠的99mTc-depreotide制备方法。方法:采用直接标记99mTc的方法,在不同的pH值(5.0、6.0和7.0)缓冲溶液和不同的环境温度(15℃、37℃和50℃)条件下,对depreotide进行标记,以纸层析法测定计算标记率并通过比较分析筛选出最佳标记条件。结果:1)同一温度条件下,pH6.0实验组标记率高于pH5.0和pH7.0两实验组。在15℃条件下,pH5.0组和pH6.0组的标记率统计分析结果为t=9.232,P<0.001;pH6.0组与pH7.0组的t=9.533,P<0.001;在37℃条件下,pH5.0组与pH6.0组的t=4.121,P<0.01;pH7.0组与pH6.0组的t=6.207,P<0.001。2)随着反应温度由15℃逐渐升高到50℃,各pH实验组的标记率不同程度地下降。结论:99mTc直接标记depreotide,在低温(<15℃)和弱酸性环境下,肿瘤标志的标记率高,稳定性好。
OBJECTIVE：To establish a simple and reliable method of making 99mTc-depreotide by direct labeling. METHODS： Depreotide was labeled with 99mTc through a direct labeling method. By comparing the labeling efficiency in different pH（s） （5.0,6.0,7.0） and temperatures（15 ℃,37 ℃,50 ℃）,the best labeling method was searched. RESULTS： 1）At the same temperatue,the labeling efficiency of the pH6.0 group was higher than that of the pH5.0 and pH7.0 groups. At the condition of 15 ℃,the labeling efficiency had a significant difference between the pH6.0 and pH5.0 groups or between the pH6.0 and pH7.0 groups （t=9.232 and 9.533,respectively,both P〈0.001）. There was a similar result at 37 ℃（t=4.121 and 6.207,respectively,both P〈0.01）. 2）The labeling efficiency of all the three different pH experiment groups reduced with the increasing temperature from 15 ℃ to 50 ℃ in this study. CONCLUSION： When depreotide is labeled with 99mTc,the temperature can not be too high（〈15 ℃） and the pH would be weak acid.
出处
《中华肿瘤防治杂志》
CAS
2009年第18期1368-1371,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
上海市卫生局科技发展基金(054035)
上海市科委基础重点项目(08JC1419700)
