摘要
目的:采用多因素整合方法建立子宫内膜异位症气滞血瘀证大鼠模型,为深入研究子宫内膜异位症气滞血瘀证的病理机制和治疗药物提供合适的动物模型。方法:在自体内膜移植法建立子宫内膜异位症疾病模型的基础上,采用药物加情志刺激等多因素干预方法造成气滞血瘀证候模型,观察模型大鼠的外部体征和行为学表现、在位和异位内膜的组织形态学表现、血管内皮因子、单胺类神经递质等的变化,对子宫内膜异位症气滞血瘀证大鼠模型进行评价。结果:大鼠建模成功率为78%;病证结合组NO含量较疾病模型组、伪手术组均增高,差异有显著性(P<0.05);病证结合组ET含量较疾病模型组增高,差异有显著性(P<0.05);与疾病模型组相比,病证结合组血清DA、NE含量均明显增高,差异有显著性(P<0.05)。结论:多因素整合方法建立的子宫内膜异位症气滞血瘀证大鼠模型基本符合子宫内膜异位症的病理特征和中医证候特点。
Objective： Using integration of multi-factor approach to establish the qi stagnancy and blood stasis endometriosis model of rats.Provide appropriate models for in-depth study of pathological mechanisms and TCM treatment of endometriosis.Methods： Based on the duplication of the rat models of endometriosis by endometrial tissue autologous transplant surgery,we used integration of multi-factor approach,such as drugs and emotional stimulation to copy the qi stagnancy and blood stasis rat models.Evaluated the rat models by observing the external and behavioral performance,tissue morphologic features of the reign and ectopic endometrial tissue,and the changes of vascular endothelial factor and monoamine neurotransmitter.Results：（1）The effective rate of the rat modeling was 78%.（2）Disease and syndrome combination group was higher in NO than that in model group and Sham-operated group,and both groups were significantly different（P〈0.05）.Disease and syndrome combination group was higher in ET than that in model group,and both groups were significantly different（P〈0.05）.（3）Compared with disease model group,disease and syndrome combination group was significantly higher in serum NE,DA,and both groups were significantly different（P〈0.05）.Conclusion： The Qi stagnancy and blood stasis endometriosis model,which is established by integration of multi-factor approach,is basically consistent with both TCM syndrome features and pathological features of Endometriosis.
出处
《中医药学报》
CAS
2012年第2期31-35,共5页
Acta Chinese Medicine and Pharmacology
基金
北京中医药大学自主选题项目(No.2009JYB22-XS001)
关键词
子宫内膜异位症
气滞血瘀
大鼠模型
Endometriosis
Qi stagnancy and blood stasis
Rat model
