摘要
目的探讨红毛五加多糖(AGP)对人乳腺癌细胞系MDA-MB-231增殖以及对细胞核转录因子p53、c-myc表达的影响。方法在DMEM培养基内加入不同浓度AGP(0.15625、0.3125、0.625、1.25、2.5 g/L)体外培养人乳腺癌细胞系MDA-MB-231 1d、3d、5d、7d后,用细胞增殖及细胞毒性检测试剂盒WST-1测定人乳腺癌细胞的增殖,倒置显微镜观察其形态变化;用免疫荧光法、免疫印迹法检测人乳腺癌细胞凋亡相关蛋白p53、c-myc的表达。结果 WST-1法显示,不同浓度AGP作用3d、5d、7d后对人乳腺癌细胞系MDA-MB-231的增殖具有抑制作用,与阴性对照组相比差异有显著性(P<0.05);抑制率与AGP剂量、作用时间呈依赖性。免疫荧光法及免疫印迹法显示,AGP作用于人乳腺癌细胞系MDA-MB-231后,其细胞的c-myc表达明显减弱,并向细胞质内聚集;而免疫印迹法显示p53表达显著增强。结论 AGP具有抑制人乳腺癌细胞系MDA-MB-231增殖的作用,这可能与AGP下调c-myc表达、上调p53表达有关。
Objective To study the effects of acanthopanax giraldii harms var bispidus boo polysaccharides （AGP） on proliferation and expression of nuclear transcription factor p53 and c-myc in human breast cancer cell line MDA- MB-231. Methods The proliferation rate of human breast cancer cell line MDA-MB-231, which was incubated by different concentration of AGP （0. 15525, 0. 3125, 0. 625, 1.25, 2.5g/L） in DMEM medium for 1, 3, 5, 7 days in vitro respectively, was studied by WST-1 cell proliferation and cytotoxicity assay kit. The changes of human breast cancer cell line MDA-MB-231 morphology were observed by an inverted microscopy. The expression of apoptosis-related proteins such as p53 and c-myc was detected by fluorescence immunoassay and Western blotting. Results WST-l assay showed that different concentration of AGP inhibited proliferation of human breast cancer cell line MDA-MB-231 treated for 3day, 5day, 7day when compared with the negative control group （P 〈0. 05）. The inhibition rate depended on AGP dose and reaction time. According to fluorescence immunoassay and Western blotting analysis, c-myc expression in cytoplasm reduced and p53 expression increased significantly after the treatment of AGP on human breast cancer cell line MDA-MB-231. Conclusion AGP has the effect of inhibiting proliferation on human breast cancer cell line MDA-MB-231. Its role may be related to reduce c-myc expression and increase p53 expression of human breast cancer cell line MDA-MB-231.
出处
《解剖学报》
CAS
CSCD
北大核心
2012年第3期387-392,共6页
Acta Anatomica Sinica
基金
上海柯莱逊生物技术有限公司资助项目(20090086)
香港解剖科学技术基金会资助项目(20090167)
北京市与中央在京高校共建项目
