摘要
目的建立Wister大鼠的深Ⅱ°烫伤创面模型,外用瘦素来观察和研究瘦素在Waster大鼠烫伤创面愈合中的作用和意义,探讨瘦素在创伤修复过程中的作用及其机制。方法选用清洁级Wistar大鼠18只,建立深Ⅱ度烫伤模型,将创面分为实验组（创面涂400 ng/ml的瘦素溶液,用PBS释稀）和对照组（外涂PBS）。观察创面愈合时间;在烫伤后第3,7,11,15,19天5个时相点测量创面的上皮化率;于伤后第7、14和21天用手术刀切取各组的伤口及周边皮肤,做HE及免疫组化染色（PCNA）,以观察创面的愈合时间和组织形态以及角质形成细胞在烫伤创面愈合过程增殖和迁移情况。结果实验组创面愈合时间快于对照组2~3 d左右,实验组愈合天数与对照组相比,差异具有显著性（P〈0.05）。在伤后7、14和21 d创缘组织HE和PCNA染色见实验组创周表皮反应性增生快,表皮层明显厚于对照组,实验组创面上皮化过程短于对照组。实验组PCNA表达与对照组在7 d和14 d时有明显差异（P〈0.05）,在21 d时没有显著差别（P〉0.05）。结论局部外用瘦素能提高深Ⅱ烫伤创面的愈合率,加快创面愈合时间,瘦素能加快深Ⅱ烫伤创面的愈合。
Objective To investigate the effect of topical application of leptin in promoting burn wound healing in rats.Methods Four parallel second-degree burn wounds induced on the back of 18 Wistar rats were divided into leptin treatment group（treated with topical application of 400 ng/ml leptin dissolved in PBS） and control group（treated with PBS）.The time of wound healing was recorded,and the wound area that was not healed was measured at 3,7,11,15,and 19 days after burns.The tissue at the peripheries of the wound was sampled at 7,14 and 21 days after burns for pathological examination with HE staining and immunohistochemistry for proliferating cell nuclear antigen（PCNA） to evaluate the proliferation of keratinocytes.Results Compared with the control group,leptin-treated wounds showed a shorter time（by 2-3 days） of wound healing,and significant differences were found between the two groups in healing at 7,11,15,and 19 days after burns.HE staining and immunohistochemistry revealed a faster rate of epidermis growth and a greater thickness of the cuticular layer in leptin-treated wounds at 7,14 and 21 days after burns.PCNA positivity in the keratinocytes was stronger in leptin-treated wounds than in the control wounds at 7 and 14 days,but no such distinct difference was noted at 21 days between the two groups.Conclusion Topical application of leptin can promote reepithelization in burn wounds to shorten the wound healing time of burns.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2012年第5期703-706,共4页
Journal of Southern Medical University
基金
江西省科技支撑计划(2008BSA05900)
