摘要
目的 :探讨rhIL 1β对人胚胎胰岛分泌IL 6以及对胰岛分泌功能的影响。 方法 :采用常规消化方法分离人胚胎胰岛并对其进行原代培养 ,加入不同浓度的rhIL 1β培养 48h取其上清 ,测定IL 6、胰岛素、胰高血糖素含量和IL 6McAb中和试验 ,对rhIL 1β刺激和对照组胰岛RNA进行IL 6RNA斑点杂交。 结果 :(1)经rhIL 1β刺激后胰岛培养上清IL 6含量显著升高 ,在一定范围内呈剂量依赖关系 (74～ 2 94mU/胰岛 ) ;(2 )IL 6McAb对上清中IL 6有很强的中和效应 ;(3)斑点杂交结果显示rhIL 1β刺激的胰岛RNA中IL 6mRNA含量升高 ;(4 )上清胰岛素和胞内胰岛素含量降低 (分别为 0 78～ 0 48IU/胰岛和 0 79～ 0 45IU/胰岛 ) ;(5 )上清胰高血糖素分泌能力明显升高 (1 0～ 3 2pg/胰岛 ) ,分泌趋势与IL 6相似。结论 :胰岛的分泌功能及产生IL 6的能力受IL
AIM: To study rhIL-1β effects on fetal islet function and IL-6 production in vitro METHODS: Islets from fetal pancreas was separated by collagenase type V (0 5 mg/mL) and cultured in vitro The islets were exposed to culture medium alone for 48 h or with different concentration of rhIL-1β The supernatants of culture of human fetal islets were assayed for IL-6, insulin and glucagon RESULTS:(1) IL-6 activity was increased 4 0 folds (74-294 mU/islet) when islets were exposed to rhIL-1β(20U/mL); (2) IL-6 McAb significantly reduced IL-6 activity in islet supernatants from control group or islet exposed to rhIL-1β treated group; (3)IL-6 mRNA in human fetal islet exposed to rhIL-1β is higher than control in dot hybridization; (4) Soluble insulin and cellular insulin within islet released to supernatants was slightly decreased (0 48～0 78 IU/islet and 0 65～0 79 IU/islet); (5) Glucagon secretion was significantly increased 3 2 folds (1 0～3 2 pg/islet) CONCLUSION: Pancreatic islets produce IL-6 is up-regulated by rhIL-1β On the other hand, Il-6 produced by the islet may act as a costimulator for autoreactive B and T lymphocytes in autoimmune diabetes
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第2期113-116,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助!(39370 6 38)
