摘要
采用免疫组化方法，观察了体外培养大鼠海马细胞对人重组白细胞介素１β（ｒｈＩＬ－１β）抗血清的免疫反应以及缺氧的影响。结果可见，体外培养的大鼠海马神经元和神经胶质细胞对抗ｒｈＩＬ－１β抗血清均呈弱阳性免疫染色反应，缺氧后免疫染色反应明显增强。经图像分析表明，缺氧后神经元和神经胶质细胞的平均光密度均较缺氧前增加，尤以缺氧２ｈ时增加最明显，继续缺氧４─１２ｈ，神经元和神经胶质细胞的平均光密度又逐渐减弱。以上结果表明，大鼠海马脑区存在抗ｒｈＩＬ－１β抗血清的免疫反应细胞；缺氧使对抗ｒｈＩＬ－１β抗血清的免疫染色反应增强，提示ＩＬ－１β与脑缺氧损伤的调控有关。
The immunoreactivity of cultured rat hippocampal cells to recombinant humaninterleukin-1β(rhIL-1β)antiserum during normoxia and anoxia was observed by means of thetechnique of immunohistochemistry. The results showed that very light stain of IL-1βimmunoreaction was seen in cell bodies and processes of both neurons and glial cells.Amarkedincrease of IL-1β immunohistochemical reactivity was found in both neurons and glial cells after1-4h exposure to anoxia. The mean optical density of immunohistochemically stained cells wassignificantly higher in anoxic cells,especially after 2h of anoxia,than those in controls by imageanalysis.Following progressive anoxia,the mean optical density of IL-1βimmunohistochemicalreactivity was gradually decreased in both neurons and glial cells.The results indicated that theIL-1β immunoreactive cells were present in hippocampus and IL-1 may be involved in the mod-ulation of anoxic injury of brain.
出处
《中国应用生理学杂志》
CAS
CSCD
1996年第2期157-161,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金
中国科学院上海生理所低氧生理开放实验室资助
