摘要
目的:建立人参多糖中糖醛酸的含量测定方法。方法:将葡萄糖和半乳糖醛酸配成一系列混合糖,考察中性糖在咔唑法和间羟基联苯法中对糖醛酸测定的干扰,选择干扰较小的方法作为人参多糖中糖醛酸的含量测定方法,并对该方法的测定条件进行优选,对方法学进行考察。结果:选择间羟基连本法作为人参多糖中糖醛酸的含量测定方法,最佳测定条件为:四硼酸钠-硫酸溶液用量为6.0 mL,加热时间为12 min,间羟基联苯加入量为80μL,显色时间为40 min,测定波长为525nm。人参多糖中糖醛酸的平均含量为29.34%。结论:该方法简便、准确,为人参多糖的的质量控制提供可借鉴的分析方法。
Objective：To establish the method for determination of the uronic acids in Polysaccharides isolated from Panax ginseng.Method： Mix the D-glucose and D-galacturonic in different proportions,and then use Carbazole method and Meta-hydroxyldiphenyl method to determine the D-galacturonic in the mixed sugar.Compare the interference of D-glucose on the determination results of D-galacturonic in this two methods.Choose the method of small disturbance as the determination method of the uronic acid in Panax ginseng Polysaccharides.Result： Using Meta-hydroxyldiphenyl method to determine the aldonic acid in Panax ginseng Polysaccharides and the average content is 29.34%.The optimized parameters were obtained as follows： the dosage of Na2B4O7-H2SO4 and m-hydroxydiphenyl was 6.0 mL and 100 μL,time for heating and color development were 12 min and 40 min respectively.The detection wavelength was 525 nm.Conclusion： Experimental results show that the method is easy and accurate,so it can be used for quality control of polysaccharides in Panax ginseng and its preparations.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期121-124,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部973计划项目(2010CB735604)
关键词
人参多糖
糖醛酸
间羟基联苯法
Panax ginseng polysaccharide
aldonic acid
meta-hydroxyldiphenyl method
