摘要
以多糖提取率、糖醛酸提取率为指标,比较水提取、酸提取和碱提取对多糖的提取效果,探索东北铁线莲多糖提取的最佳方法和工艺。分别以水、0.1mol/L盐酸、0.1mol/L氢氧化钠溶液、100℃水浴提取铁线莲多糖,苯酚-浓硫酸法测定东北铁线莲多糖含量,间羟基联苯法测定糖醛酸含量。结果,水提取、酸提取、碱提取东北铁线莲多糖含量分别为50.947%、29.064%、22.083%,糖醛酸含量分别为0.952%、38.903%、6.588%。水提取法的多糖提取率远远高于酸提取与碱提取,酸提取的糖醛酸提取率高于水提取与碱提取。
Extraction technique of polysaccharides from Clematis manshurica Rupr .was optimized .Compared with the extraction rate of polysac-charides and uronic acid by water ,acid and alkali .Methods ：extraction of the polysaccharide from Clematis mandshurica Rupr .with 0.1mol/L HCL ,0.1mol/L NaOH and 100℃ water bath respectively .Determination of the contents of polysaccharide in Clematis mandshurica Rupr .phe-nol by sulfuric-acid method with the method of M -hydroxydiphenyl to Determination of the contents of polysaccharide in Clematis mandshuri-ca Rupr .As a result that the extracted contents of polysaccharides were 50.947% ,29.064% ,22.083% .and the extracted contents of uronic acid by acid solution were 0.952% 、38.903% 、6.588% in Clematis manshurica Rupr .The extraction rate of water is higher in polysaccharides from Clematis manshurica Rupr .and the extraction rate of uronic acid with acid solution is higher than water and alkali solution .The extraction technique operation is simple and good stability ,It is a well determination technique in the contents of polysaccharide in Clematis mandshuric Rupr .
出处
《特产研究》
2014年第4期60-62,共3页
Special Wild Economic Animal and Plant Research
关键词
东北铁线莲
多糖
提取方法
polysaccharides
extracting technology
