摘要
目的:分析大鼠黄体生成素(LH)表达的受体后信号转导机制。方法:促性腺激素(GTH)细胞内蛋白激酶C(PKC)兴奋或抑制后,用促性腺激素释放激素(GnRH)脉冲刺激,然后用实时荧光定量PCR方法测定细胞LH的β亚基(LHβ)mRNA的表达量,并与空白组比较。结果:LHβmRNA随着PKC活性的升高而显著升高,随着PKC活性的降低而显著降低。结论:GnRH脉冲刺激引起LHβmRNA表达,其受体后的信号转导是PKC-Ca2+途径。
Objective： To analyze the post-receptor signal transduction mechanism of the expression of luteinizing hormone（LH） in the rats. Methods： The gonadotropic hormones（GTH） cells were stimulated by gonadotrophin hormone-releasing hormone（GnRH） after protein kinase C（PKC） in them was excited or inhibited. LHβ mRNA was done by Real-time quantitative PCR（RTqPCR）, which was compared with the blank comparison group. Re suits： LHβmRNA rises significantly with the rise of PKC and fall significantly with the falling of PKC. Conclusion： The cells stimulated by GnRH impulse responded LHβmRNA and the post-receptor signal transduction pathway is PKC-Ca2＋.
出处
《生物技术通讯》
CAS
2012年第2期195-197,共3页
Letters in Biotechnology
基金
国家自然科学基金(30571356)
青岛农业大学高层次人才启动基金(630810)
实验技术课题(SYJK09-05)
教学研究课题(XJG0906)
广州中医药大学博士后科研启动金(B3YH1008)
关键词
促性腺激素释放激素
黄体生成素
蛋白激酶C
促性腺激素
信号转导
大鼠
gonadotrophin hormone-releasing hormone
luteinizing hormone
protein kinase C
gonadotropic hor-mones
regulation
rats
