摘要
背景单核细胞趋化蛋白-1（MCP-1）是趋化因子家族中的主要成员之一，在肿瘤、炎症和糖尿病视网膜病变（DR）等新生血管性疾病中起着重要的作用，但关于MCP-1在氧诱导视网膜病变（OIR）发生过程中的表达及其作用的研究鲜有报道。目的观察OIR小鼠视网膜中MCP-1的表达，探讨MCP-1在视网膜血管发育和视网膜新生血管形成过程中的作用。方法SPF级C57BL／6J小鼠120只，按随机数字表法随机分为OIR组和正常对照组，每组60只。OIR组将出生后7d的新生小鼠在体积分数75％氧环境下饲养5d，然后返回正常大气环境中；正常对照组小鼠始终置于正常大气环境中饲养。OIR组和正常对照组分别在出生后5、7、12、14、17、21d随机抽取10只小鼠，摘除右眼球，采用免疫组织化学法检测MCP-1蛋白在小鼠视网膜中的表达情况，采用逆转录-聚合酶链反应（RT—PCR）法检测MCP-1mRNA在小鼠视网膜中的表达。结果正常对照组小鼠在出生后的第5天即有MCP-1在视网膜的内核层和节细胞层表达，12d时表达MCP-1的阳性细胞数达到高峰，其他日龄时呈基线表达。OIR组12d时表达MCP-1的阳性细胞数轻度增多，14d时阳性细胞数显著增多，之后迅速下降至正常水平。正常对照组在5d时可检测到MCP-1mRNA表达，12d时显著上调，之后随小鼠日龄的增加，MCP-1mRNA表达迅速下降，14、17、21d表达基本呈基线水平。OIR组12d时，MCP-1mRNA较正常对照组下降，在新生血管形成关键期，即14d时表达显著上调，之后迅速下降至正常水平。OIR组和正常对照组间比较采用完全随机分组的两因素方差分析，F分组=5．230，P=0．028；F日龄6．898，P=0．001。结论小鼠视网膜发育过程始终伴随着MCP-1的表达，MCP-1的表达上调可能与小鼠视网膜血管发育和OIR模型中视网膜新生血管的形成密切相关。
Background Monocyte chemotactic protein-1 （MCP-1）plays an important role inflammation,diabetic retinopathy and other neovascular disease, but the expression and the role of oxygen induced retinopathy（OIR） model have rarely been reported. Objective This study was to expression of MCP-1 in the retina development of newborn mouse and in mouse models with OIR. in the tumor, MCP-1 in the investigate the Methods C57BL/6J newborn mice were divided into two groups and 60 mice in each group. Mice in OIR group were exposed to 75% oxygen for 5 days and then to room air. All mice in normal control group exposed to room air only. Ten mice in each group were randomly chosen and sacrificed at postnatal 5,7,12,14,17,21 days. The expression of MCP-1 in mouse retina was detected with the method of immunohistoehemistry and reverse transcription polymerase chain reaction（RT-PCR）. Results MCP-1 positive cells were seen in normal mouse retina. Up-regulation of MCP-1 positive cells was detected both in 12 days in normal control group and in 14 days in OIR group. MCP-1 mRNA was detected in mouse retina at 5 days,and a transient up-regulation of MCP-1 mRNA was observed in 12 days in normal control group. MCP-1 mRNA in OIR group significantly increased in 14 days in comparison with the normal control group（ P = 0. 028, P = 0. 001 ）. Conclusions Expression of MCP-1 is detectable in whole retinal development procession of mice. A transient up-regulation of MCP-1 expression is detected in the critical period of retinal vascular development in mice models with OIR,which is closely related to the retinal vascular development and progression of retinal new vessels.
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2012年第4期293-296,共4页
Chinese Journal Of Experimental Ophthalmology
基金
国家自然科学基金青年基金（30700919）、北京市优秀人才培养资助项目（20100003034000006）
