摘要
目的研究单核细胞趋化蛋白-1(Monocyte chemoattractant protein-1,MCP-1)在兔糖尿病皮肤缺损模型愈合进程中的表达水平,探讨其在糖尿病皮肤愈合中的作用.方法制作兔糖尿病模型,通过免疫组化和ELISA的方法研究MCP-1在兔糖尿病皮肤缺损模型愈合进程中的局部表达及血清中水平.结果免疫组化染色提示MCP-1在糖尿病组中各时间点位表达均高于空白对照组.外周血中造模后3 d和1周时MCP-1含量糖尿病组与空白组比较有统计学意义(P<0.05),2周和4周时无统计学意义(P>0.05).结论 MCP-1可能在糖尿病皮肤缺损修复愈合紊乱的炎性反应中发挥作用,最终干扰愈合.
Objective To study the expression of MCP-1 in the diabetes mellitus skin defect healing, then discuss its effect. Methods The diabetes mellitus models were established, ＇and the expression of MCP-1 was detected by immunohistochemical staining and Elisa. Results The immunohistochemistry stain showed that the expression of MCP-1 in DM group was higher than that in control group at all time. And the serum value of MCP-1 in DM group had statistical significance （P 〈 0.05） at 3 days or 1 week, but that in 2 weeks and 4 weeks was no statistical significance （P 〈 0.05） . Conclusion The MCP-1 might play a role in the healing of diabetes mellitus skin defect through mediate abnormal inflammatory response.
出处
《昆明医科大学学报》
CAS
2013年第9期51-54,共4页
Journal of Kunming Medical University
