摘要
使用不连续PAGE法,分析了10尾准噶尔雅罗鱼(Leuciscus merzbacheri)眼睛、鳃、皮、背部肌肉、鳍和肝胰脏6种组织的10种同工酶(LDH,CCO,EST,CAT,POD,ME,MDH,G6PD,GDH,ADH)的差异表达,并对部分同工酶基因位点及表达酶谱表型进行了分析,以期为其种质资源保护和开发以及遗传育种等方面的研究提供基础资料。结果显示,10种同工酶中9种在6种组织中出现了明显的组织差异性,仅CCO在6种组织中的差异性较小。在对准噶尔雅罗鱼的10种同工酶的遗传多样性分析中,共记录到了21个基因位点,其中Est-1、Me-B、s-MDH、G6pd-A、G6pd-B和Adh-A为多态性基因位点。多态位点比例为:P=6/21=28.57%。
Polyacrylamide gel electrophoresis was used to detect the expression of ten isozymes （LDH, CCO, EST, CAT, POD, ME, MDH, G6PD, GDH, and ADH） in six different tissues, including eye, gill, skin, muscle, fin, and liver. Part of isozymes gene loci and zymograms were analyzed for providing the foundation for conservation and development of germplasm resources and genetic breeding. The results showed that the electrophoresis patterns of isozymes exhibited an apparent tissue-speclficity except for CCO which showed no evident difference in 6 tissues examined. Biochemical genetic analysis showed that the ten isozymes were coded by 21 gene loci, six of which were found to be polymorphic. Finally, based on the zymograms, six isozymes including Est1, MeB, sMDH, G6pdA, C,6pdB and AdhA were analyzed genetically for their subunit structure, coding gene loci and numbers as well as their specific expression, and the proportion of polymorphie loci was P =6/21 =28.57%.
出处
《动物学杂志》
CAS
CSCD
北大核心
2012年第2期105-111,共7页
Chinese Journal of Zoology
基金
国家自然科学基金项目(No.30860215)
关键词
准噶尔雅罗鱼
同工酶
组织差异性
Leuciscus merzbacheri
Isozymes
Tissue specificity
