摘要
在水温为(23±1)℃下,将初始体质量为(3.37±0.03)g的红鲫Carassius auratus 180尾,随机分为4组,每组设3个重复,每个重复15尾,饲养在200 L聚乙烯水槽中,投喂在7%肉粉(MM组)和8%玉米蛋白粉(CGM组)中添加0.1%蛋氨酸(MM+CAA组)和0.1%赖氨酸(CGM+CAA组)的饲料56 d,研究上述2种蛋白源饲料中两种限制性氨基酸对红鲫生长、免疫及抗氧化能力的影响。结果表明:蛋白源与氨基酸对红鲫摄食率(FI)、肌肉总必需氨基酸(∑IDAA)和总氨基酸(∑AA)含量及肠道淀粉酶(AMS)活性无显著交互作用(P>0.05);肉粉蛋白源饲料中添加0.1%的两种限制性氨基酸后,与其余各组相比,显著提高了红鲫的特定生长率(SGR)、增重率(WGR)、肥满度(CF)、肌肉蛋氨酸(Met)和赖氨酸(Lys)含量、肠道脂肪酶(LPS)和蛋白酶(PRO)活性、肝脏酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性(P<0.05);显著降低了肝脏丙二醛(MDA)含量(P<0.05),而饲料系数(FCR)显著低于玉米蛋白粉为蛋白源未添加限制性氨基酸组。综上所述,在本实验条件下,肉粉蛋白源饲料中添加蛋氨酸和赖氨酸可以显著改善红鲫的生长、消化、免疫、抗氧化指标以及肌肉中限制性氨基酸水平,提高饲料利用率同时降低饲料成本。
A total of 180 individuals of red crucian carp Carassius auratus with initial body weight of(3.37±0.03)g were randomly divided into 4 groups with 3 replicates per group,reared in a 200 L polyethylene tank and fed diets containing meat meal protein source(MM group)and corn meal protein source(CGM group)supplemented with two limited amino acids methionine and lysine at a dose of 0.1%and 0.1%(MM+CAA group and CGM+CAA group)at a water temperature of(23±1)℃for 56 d to evaluate effects of limited amino acids supplementation in different protein sources diets on growth performance,immunity,and antioxidant capacity of red crucian carp.The results showed that there were significantly higher specific growth rate(SGR),weight gain rate(WGR),condition factor(CF),the contents of muscular methionine(Met)and lysine(Lys),and activities of intestinal lipase(LPS)and protease(PRO),liver acid phosphatase(ACP),alkaline phosphatase(AKP),catalase(CAT),and superoxide dismutase(SOD),especially in meat meal protein group with limited amino acids supplementation(P<0.05)in the fish fed the diets containing meat meal protein source(MM group)and 0.1%limited amino acids than those in other groups,without significant interaction between protein sources and amino acids on total indispensable amino acid contents(∑IDAA),total amino acid contents(∑AA),feeding intake(FI),and amylase activity(AMS)(P>0.05)than those in other groups.Meanwhile,significant decrease in the content of liver malondialdehyde(MDA)was observed in the fish fed the diets containing meat meal protein source(MM group)and 0.1%limited amino acids(P<0.05).The food conversion ratio(FCR)was shown to be significantly decreased in the fish fed the diets containing meat meal protein source and 0.1%limited amino acids than that in the fish fed the diets containing corn meal protein group without amino acids supplementation(P<0.05).To sum up,the addition of methionine and lysine to the practical feed with meat powder as the main protein source led to significantly improve the growth,
作者
石立冬
卫力博
翟浩杰
牟玉双
侯仁爽
任同军
韩雨哲
SHI Lidong;WEI Libo;ZHAI Haojie;MU Yushuang;HOU Renshuang;REN Tongjun;HAN Yuzhe(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;Key Laboratory of Fish Applied Biology and Aquaculture in North China,Dalian Ocean University,Dalian 116023,China)
出处
《水产学杂志》
CAS
北大核心
2021年第2期56-64,共9页
Chinese Journal of Fisheries
基金
辽宁省海洋产业技术研究院项目(2018-CY-30)
大连市青年科技之星
大连海洋大学“蔚蓝英才”人才计划项目.
关键词
蛋白源
限制性氨基酸
生长
红鲫
protein source
limited amino acid
growth performance
Carassius auratus
