摘要
采用不连续聚丙烯酰胺垂直板凝胶电泳的方法,对广东鲂(Megalobrama terminalis)、(Hemiculter leucisculus)、鲮(Cirrhina cuvier)、草鱼(Ctenopharyngodon idellus)、青鱼(Mylopharyngodon piceus)、鳜(Siniperca chuatsi)6种不同食性鱼的肝脏、肌肉、心脏、眼、鳃、肾等6种新鲜组织的酯酶(EST)及α-淀粉酶(α-AMY)同工酶进行了比较研究。结果表明:这6种不同食性鱼6种组织中均存在EST和α-AMY同工酶的分布,他们具有明显的组织特异性;在这些鱼中,EST同工酶的种类和表达程度较α-AMY同工酶都占优势;肝脏和肾脏中EST和α-AMY同工酶的活性高于其他组织;EST和α-AMY同工酶在不同食性鱼中表现出明显的差异。通过对EST和α-AMY同工酶的特性分析,可了解并研究鱼类当前的食性状态,进一步掌握鱼类栖息环境的营养源与生态状况。
By means of poyacrylamid gel electrophoresis, two isozymes （EST, α-AMY）in liver, muscle, heart, eye, gill, kidney of six kinds of adult fish including Megalobrama terminalis, Hemiculter leucisculus, Cirrhina cuvier, Ctenopharyngodon idellus, Mylopharyngodon piceus, Siniperca chuatsi were studied. The results showed that the EST and α-AMY isozymes were very complicated and all the isozyme phenotypes exhibited tissue-specificity. The species and expression degree of EST isozyme was higher than that of α-AMY isozyme in these fishes. The activities of EST isozyme and α-AMY isozyme in liver and kidney were higher than those in other organs. There exited difference in different feeding fishes, so analysis on the characteristic of EST and α-AMY isozymes could reveal the current status of feeding habitat, know the nutrition sources and ecological condition of fish habitats.
出处
《广东农业科学》
CAS
2015年第8期89-94,共6页
Guangdong Agricultural Sciences
基金
国家公益性行业(农业)科研专项(2013 03048)
广西自然科学基金重大项目(2013GXNSFEA053003)
科技部社会公益项目(2005DIB3J023)
